Abstract
Oxygen is the terminal electron acceptor in the mitochondrial electron transport chain and therefore is required for the generation of energy through oxidative phosphorylation. In environments of decreased oxygen levels (hypoxia), organisms have developed an adaptive response through the activation of the hypoxia-inducible transcription factor (HIF) to maintain their energetic demand. In order to sense hypoxic environments, cells have developed oxygen-sensing machinery that allows for the activation of HIF. The mitochondrial electron transport chain is required for the oxygen-sensing pathway. This chapter outlines methods used to explore the role of the electron transport chain and a by-product of electron transport, reactive oxygen species, in oxygen sensing.
Original language | English (US) |
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Title of host publication | Oxygen Biology and Hypoxia |
Editors | Helmut Sies, Bernhard Brune |
Pages | 447-461 |
Number of pages | 15 |
DOIs | |
State | Published - 2007 |
Publication series
Name | Methods in Enzymology |
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Volume | 435 |
ISSN (Print) | 0076-6879 |
Funding
This work was supported in part by National Institutes of Health Grants (GM60472‐07) to Navdeep S. Chandel. Eric Bell is supported by the American Heart Association Grant (0515563Z).
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry