@article{f7dbab625bfb4cd5b04c934f5cc68859,
title = "Genome mining and metabolomics uncover a rare D-capreomycidine containing natural product and its biosynthetic gene cluster",
abstract = "We report the metabolomics-driven genome mining of a new cyclic-guanidino incorporating non-ribosomal peptide synthetase (NRPS) gene cluster and full structure elucidation of its associated hexapeptide product, faulknamycin. Structural studies unveiled that this natural product contained the previously unknown (R,S)-stereoisomer of capreomycidine, D-capreomycidine. Furthermore, heterologous expression of the identified gene cluster successfully reproduces faulknamycin production without an observed homologue of VioD, the pyridoxal phosphate (PLP)dependent enzyme found in all previous L-capreomycidine biosynthesis. An alternative NRPS-dependent pathway for D-capreomycidine biosynthesis is proposed.",
author = "Kelleher, {Neil L.} and Thomson, {Regan J.} and Tryon, {James H.} and Rote, {Jennifer C.} and Li Chen and Robey, {Matthew T.} and Vega, {Marvin M.} and Phua, {Wan Cheng} and Metcalf, {William W.} and Ju, {Kou San}",
note = "Funding Information: The research was supported by the National Institutes of Health under Award Numbers AT009143 (to N.L.K. and R.J.T.) and D012016 (Integrated Molecular Structure Education and Research Center at Northwestern University). J.C.R gratefully acknowledges the National Science Foundation for the award of a Graduate Research Fellowship. W.C. P gratefully acknowledges the award of an Undergraduate Research Grant from Northwestern University. K.S.J. acknowledges support from the Center for Applied Plant Sciences (Scientific Team Grant BPBFP) at The Ohio State University. Publisher Copyright: {\textcopyright} 2020 American Chemical Society",
year = "2020",
month = nov,
day = "20",
doi = "10.1021/acschembio.0c00663",
language = "English (US)",
volume = "15",
pages = "3013--3020",
journal = "ACS Chemical Biology",
issn = "1554-8929",
publisher = "American Chemical Society",
number = "11",
}