Genome-wide chromatin maps derived from limited numbers of hematopoietic progenitors

Mazhar Adli, Jiang Zhu, Bradley E. Bernstein

Research output: Contribution to journalArticlepeer-review

124 Scopus citations


Current methods for whole-genome mapping of protein-DNA interactions, performed by coupling chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq), require large amounts of starting materials, which precludes their application to rare cell types. Here we combine a high-sensitivity ChIP assay with a new library preparation procedure to map histone modifications in as few as 10,000 cells. We used the technique to characterize mouse hematopoietic progenitors and thereby gain insight into their developmental program.

Original languageEnglish (US)
Pages (from-to)615-618
Number of pages4
JournalNature Methods
Issue number8
StatePublished - Aug 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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