Genome-wide survey of protein kinases required for cell cycle progression

M. Bettencourt-Dias*, R. Giet, R. Sinka, A. Mazumdar, W. G. Lock, F. Balloux, P. J. Zafiropoulos, S. Yamaguchi, S. Winter, R. W. Carthew, M. Cooper, D. Jones, L. Fronz, D. M. Glover

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

289 Scopus citations


Cycles of protein phosphorylation are fundamental in regulating the progression of the eukaryotic cell through its division cycle. Here we test the complement of Drosophila protein kinases (kinome) for cell cycle functions after gene silencing by RNA-mediated interference. We observed cell cycle dysfunction upon downregulation of 80 out of 228 protein kinases, including most kinases that are known to regulate the division cycle. We find new enzymes with cell cycle functions; some of these have family members already known to phosphorylate microtubules, actin or their associated proteins. Additionally, depletion of several signalling kinases leads to specific mitotic aberrations, suggesting novel roles for familiar enzymes. The survey reveals the inter-digitation of systems that monitor cellular physiology, cell size, cellular stress and signalling processes with the basic cell cycle regulatory machinery.

Original languageEnglish (US)
Pages (from-to)980-987
Number of pages8
Issue number7020
StatePublished - Dec 23 2004

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Genome-wide survey of protein kinases required for cell cycle progression'. Together they form a unique fingerprint.

Cite this