Genomic aberrations in myeloid sarcoma without blood or bone marrow involvement: Characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays

M. Kamran Mirza; Madina Sukhanova; Friedrich Stölzel; Kenan Onel; Richard A. Larson; Wendy Stock; Gerhard Ehninger; Friederike Kuithan; Klaus Zöphel; Poluru Reddy; Loren Joseph; Gordana Raca

doi:10.1016/j.leukres.2014.05.004

Genomic aberrations in myeloid sarcoma without blood or bone marrow involvement: Characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays

M. Kamran Mirza, Madina Sukhanova, Friedrich Stölzel, Kenan Onel, Richard A. Larson, Wendy Stock, Gerhard Ehninger, Friederike Kuithan, Klaus Zöphel, Poluru Reddy, Loren Joseph, Gordana Raca^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Myeloid sarcoma (MS) is a presentation of acute myeloid leukemia (AML) as a tumor mass outside of the bone marrow. Viable cells from MS are frequently unavailable for cytogenetic studies. We therefore investigated whether chromosomal microarray analysis (CMA) using formalin-fixed paraffin-embedded (FFPE) tissues can detect clinically important genetic abnormalities in MS. CMA successfully identified genomic aberrations in six cases of MS, and in two cases it revealed multiple abnormalities equivalent to a complex karyotype, thus predicting a poor outcome. CMA using FFPE material is therefore a feasible and clinically applicable approach for detection of prognostically significant genomic abnormalities in MS.

Original language	English (US)
Pages (from-to)	1091-1096
Number of pages	6
Journal	Leukemia Research
Volume	38
Issue number	9
DOIs	https://doi.org/10.1016/j.leukres.2014.05.004
State	Published - Sep 2014

Keywords

Chromosomal microarrays
Cytogenetics
Myeloid sarcoma

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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Mirza, M. K., Sukhanova, M., Stölzel, F., Onel, K., Larson, R. A., Stock, W., Ehninger, G., Kuithan, F., Zöphel, K., Reddy, P., Joseph, L., & Raca, G. (2014). Genomic aberrations in myeloid sarcoma without blood or bone marrow involvement: Characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays. Leukemia Research, 38(9), 1091-1096. https://doi.org/10.1016/j.leukres.2014.05.004

Mirza, M. Kamran ; Sukhanova, Madina ; Stölzel, Friedrich ; Onel, Kenan ; Larson, Richard A. ; Stock, Wendy ; Ehninger, Gerhard ; Kuithan, Friederike ; Zöphel, Klaus ; Reddy, Poluru ; Joseph, Loren ; Raca, Gordana. / Genomic aberrations in myeloid sarcoma without blood or bone marrow involvement : Characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays. In: Leukemia Research. 2014 ; Vol. 38, No. 9. pp. 1091-1096.

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title = "Genomic aberrations in myeloid sarcoma without blood or bone marrow involvement: Characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays",

abstract = "Myeloid sarcoma (MS) is a presentation of acute myeloid leukemia (AML) as a tumor mass outside of the bone marrow. Viable cells from MS are frequently unavailable for cytogenetic studies. We therefore investigated whether chromosomal microarray analysis (CMA) using formalin-fixed paraffin-embedded (FFPE) tissues can detect clinically important genetic abnormalities in MS. CMA successfully identified genomic aberrations in six cases of MS, and in two cases it revealed multiple abnormalities equivalent to a complex karyotype, thus predicting a poor outcome. CMA using FFPE material is therefore a feasible and clinically applicable approach for detection of prognostically significant genomic abnormalities in MS.",

keywords = "Chromosomal microarrays, Cytogenetics, Myeloid sarcoma",

author = "Mirza, {M. Kamran} and Madina Sukhanova and Friedrich St{\"o}lzel and Kenan Onel and Larson, {Richard A.} and Wendy Stock and Gerhard Ehninger and Friederike Kuithan and Klaus Z{\"o}phel and Poluru Reddy and Loren Joseph and Gordana Raca",

note = "Funding Information: This work was funded by the American Cancer Society Institutional Research Grant (# IRG-58-004 ) and the University of Chicago Comprehensive Cancer Center Support Grant (# P30 CA14599 ). ",

year = "2014",

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doi = "10.1016/j.leukres.2014.05.004",

language = "English (US)",

volume = "38",

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Mirza, MK, Sukhanova, M, Stölzel, F, Onel, K, Larson, RA, Stock, W, Ehninger, G, Kuithan, F, Zöphel, K, Reddy, P, Joseph, L & Raca, G 2014, 'Genomic aberrations in myeloid sarcoma without blood or bone marrow involvement: Characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays', Leukemia Research, vol. 38, no. 9, pp. 1091-1096. https://doi.org/10.1016/j.leukres.2014.05.004

Genomic aberrations in myeloid sarcoma without blood or bone marrow involvement : Characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays. / Mirza, M. Kamran; Sukhanova, Madina; Stölzel, Friedrich; Onel, Kenan; Larson, Richard A.; Stock, Wendy; Ehninger, Gerhard; Kuithan, Friederike; Zöphel, Klaus; Reddy, Poluru; Joseph, Loren; Raca, Gordana.

In: Leukemia Research, Vol. 38, No. 9, 09.2014, p. 1091-1096.

Research output: Contribution to journal › Article › peer-review

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T1 - Genomic aberrations in myeloid sarcoma without blood or bone marrow involvement

T2 - Characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays

AU - Mirza, M. Kamran

AU - Sukhanova, Madina

AU - Stölzel, Friedrich

AU - Onel, Kenan

AU - Larson, Richard A.

AU - Stock, Wendy

AU - Ehninger, Gerhard

AU - Kuithan, Friederike

AU - Zöphel, Klaus

AU - Reddy, Poluru

AU - Joseph, Loren

AU - Raca, Gordana

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N2 - Myeloid sarcoma (MS) is a presentation of acute myeloid leukemia (AML) as a tumor mass outside of the bone marrow. Viable cells from MS are frequently unavailable for cytogenetic studies. We therefore investigated whether chromosomal microarray analysis (CMA) using formalin-fixed paraffin-embedded (FFPE) tissues can detect clinically important genetic abnormalities in MS. CMA successfully identified genomic aberrations in six cases of MS, and in two cases it revealed multiple abnormalities equivalent to a complex karyotype, thus predicting a poor outcome. CMA using FFPE material is therefore a feasible and clinically applicable approach for detection of prognostically significant genomic abnormalities in MS.

AB - Myeloid sarcoma (MS) is a presentation of acute myeloid leukemia (AML) as a tumor mass outside of the bone marrow. Viable cells from MS are frequently unavailable for cytogenetic studies. We therefore investigated whether chromosomal microarray analysis (CMA) using formalin-fixed paraffin-embedded (FFPE) tissues can detect clinically important genetic abnormalities in MS. CMA successfully identified genomic aberrations in six cases of MS, and in two cases it revealed multiple abnormalities equivalent to a complex karyotype, thus predicting a poor outcome. CMA using FFPE material is therefore a feasible and clinically applicable approach for detection of prognostically significant genomic abnormalities in MS.

KW - Chromosomal microarrays

KW - Cytogenetics

KW - Myeloid sarcoma

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