Genomic analysis using high-density single nucleotide polymorphism-based oligonucleotide arrays and multiplex ligation-dependent probe amplification provides a comprehensive analysis of INI1/SMARCB1 in malignant rhabdoid tumors

Eric M. Jackson, Angela Jae Waanders, Xiaowu Gai, Hakon Hakonarson, Alexander R. Judkins, Laura Tooke, Juan Carlos Perin, Hongbo Xie, Tamim H. Shaikh, Jaclyn A. Biegel

Research output: Contribution to journalArticlepeer-review

145 Scopus citations

Abstract

Purpose: A high-resolution genomic profiling and comprehensive targeted analysis of INI1ISMARCB1 of a large series of pediatric rhabdoid tumors was done. The aim was to identify regions of copy number change and loss of heterozygosity (LOH) that might pinpoint additional loci involvedin the development or progression of rhabdoid tumors and define the spectrum of genomic alterations of INI1 in this malignancy. Experimental Design: A multiplatform approach using Illumina single nucleotide polymorphism-based oligonucleotide arrays, multiplex ligation-dependent probe amplification, fluorescence in situ hybridization, and coding sequence analysis was used to characterize genome-wide copy number changes, LOH, and genomic alterations of INI1/SMARCB1 in a series of pediatric rhabdoid tumors. Results: The biallelic alterations of INI1 that ledto inactivation were elucidated in 50 of 51 tumors. INI1 inactivation was shown by a variety of mechanisms, including deletions, mutations, and LOH. The results from the array studies highlighted the complexity of rearrangements of chromosome 22 comparedwith the low frequency of alterations involving the other chromosomes. Conclusions: The results from the genome-wide single nucleotide polymorphism array analysis suggest that INI1 is the primary tumor suppressor gene involvedin the development of rhabdoid tumors with no second locus identified. In addition, we did not identify hotspots for the breakpoints in sporadic tumors with deletions of chromosome 22q11.2. By employing a multimodality approach, the wide spectrum of alterations of INI1 can be identified in the majority of patients, which increases the clinical utility of molecular diagnostic testing.

Original languageEnglish (US)
Pages (from-to)1923-1930
Number of pages8
JournalClinical Cancer Research
Volume15
Issue number6
DOIs
StatePublished - Mar 15 2009

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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