Genomic changes in synthetic Arabidopsis polyploids

Andreas Madlung, Anand P. Tyagi, Brian Watson, Hongmei Jiang, Trevor Kagochi, Rebecca W. Doerge, Robert Martienssen, Luca Comai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

252 Scopus citations

Abstract

Polyploids are common and arise frequently by genome duplication (autopolyploids) or interspecific hybridization (allopolyploids). Neoallopolyploids display sterility, lethality, phenotypic instability, gene silencing and epigenetic changes. Little is known about the molecular basis of these phenomena, and how much genomic remodeling happens upon allopolyploidization. Extensive genomic remodeling has been documented in wheat, but little remodeling occurs in cotton. Newly synthesized Arabidopsis allopolyploids, which display phenotypic instability and low fertility, displayed several, possibly related mechanisms that can remodel genomes. We detected transcriptional activity of several transposons although their transposition was limited. One represents a new family of conditionally active En-Spm-like transposons of Arabidopsis thaliana, which underwent remodeling of CG methylation upon allopolyploidization. A random amplified fragment length polymorphism survey suggested remodeling at few, specific loci. Meiotic analyses revealed the appearance of chromosomal fragments in a substantial fraction of anther meiocytes. In several individuals produced by hybrids between the synthetic and a natural allopolyploid pollen viability inversely correlated with meiotic instability. Activity of selected DNA transposons and the possibly related chromosomal breaks could cause changes by inducing translocations and rearrangements.

Original languageEnglish (US)
Pages (from-to)221-230
Number of pages10
JournalPlant Journal
Volume41
Issue number2
DOIs
StatePublished - Jan 2005

Keywords

  • Allopolyploidy
  • Epigenetics
  • Hybrid genome
  • Meiosis
  • Transposon

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Genomic changes in synthetic Arabidopsis polyploids'. Together they form a unique fingerprint.

Cite this