Genomic profiling of rice sperm cell transcripts reveals conserved and distinct elements in the flowering plant male germ lineage

Scott D. Russell*, Xiaoping Gou, Chui E. Wong, Xinkun Wang, Tong Yuan, Xiaoping Wei, Prem L. Bhalla, Mohan B. Singh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Genomic assay of sperm cell RNA provides insight into functional control, modes of regulation, and contributions of male gametes to double fertilization. Sperm cells of rice (Oryza sativa) were isolated from field-grown, disease-free plants and RNA was processed for use with the full-genome Affymetrix microarray. Comparison with Gene Expression Omnibus (GEO) reference arrays confirmed expressionally distinct gene profiles. A total of 10732 distinct gene sequences were detected in sperm cells, of which 1668 were not expressed in pollen or seedlings. Pathways enriched in male germ cells included ubiquitin-mediated pathways, pathways involved in chromatin modeling including histones, histone modification and nonhistone epigenetic modification, and pathways related to RNAi and gene silencing. Genome-wide expression patterns in angiosperm sperm cells indicate common and divergent themes in the male germline that appear to be largely self-regulating through highly up-regulated chromatin modification pathways. A core of highly conserved genes appear common to all sperm cells, but evidence is still emerging that another class of genes have diverged in expression between monocots and dicots since their divergence. Sperm cell transcripts present at fusion may be transmitted through plasmogamy during double fertilization to effect immediate post-fertilization expression of early embryo and (or) endosperm development.

Original languageEnglish (US)
Pages (from-to)560-573
Number of pages14
JournalNew Phytologist
Volume195
Issue number3
DOIs
StatePublished - Aug 2012

Keywords

  • Angiosperm sperm cells
  • Chromatin modeling
  • Expression profiling
  • Gamete transcriptome
  • Male gamete expression
  • Male germ unit
  • Oryza sativa

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Fingerprint Dive into the research topics of 'Genomic profiling of rice sperm cell transcripts reveals conserved and distinct elements in the flowering plant male germ lineage'. Together they form a unique fingerprint.

Cite this