GLI activates transcription through a herpes simplex viral protein 16- like activation domain

Joon Won Yoon, Cheng Zheng Liu, Jian Tao Yang, Rachel Swart, Philip Iannaccone, David Walterhouse*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Three proteins have been identified in mammals, GLI, GLI2, and GLI3, which share a highly conserved zinc finger domain with Drosophila Cubitus interruptus and are believed to function as transcription factors in the vertebrate Sonic hedgehog-Patched signaling pathway. To understand the role GLI plays in the Sonic hedgehog-Patched pathway and mechanisms of GLI- induced transcriptional regulation, we have characterized its transcriptional regulatory properties and contributions of specific domains to transcriptional regulation. We have demonstrated that GLI activates expression of reporter constructs in HeLa cells in a concentration-dependent manner through the GLI consensus binding motif and that a GAlA binding domain-GLI fusion protein activates reporter expression through the GAL4 DNA binding site. GLI-induced transcriptional activation requires the carboxyl- terminal amino acids 1020-1091, which includes an 18-amino acid region highly similar to the α-helical herpes simplex viral protein 16 activation domain, including the consensus recognition element for the human TFIID TATA box- binding protein-associated factor TAF(II)31 and conservation of all three amino acid residues believed to contact directly chemically complementary residues in TAF(II)31. The presence of this region in the GLI activation domain provides a mechanism for GLI-induced transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)3496-3501
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number6
DOIs
StatePublished - Feb 6 1998

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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