We have analyzed the genomic DNA regulating the testis specific transcription of lac t ate dehydrogenase c (Idh-f ), a member of the lactate dehydrogenase gene family. Deletion analyses revealed thai a 60 bp core promoter sequence was sufficient to maintain testis specificity in an in vitro transcription system. A 31 bp palindromic sequence containing a functional TA TA box and the transcription start site is involved in both positive and negative regulation of transcription. Consensus regulatory elements include a GO box at -70 bp, a OCA AT box at -592 bp, and a recognition site for the DNA binding protein GLI at -246 bp -V (o the transcription start site. We report here that the DNA binding domain of Ci (Drosophila GLI homolog) binds to the mouse Idh-c promoter. Deletion of a 335 bp promoter fragment containing the GLI binding site reduces in vitro transcription in testis nuclear extract by a factor of three. This decrease may be due to the loss of the GLI binding site. GLI is a transcription factor containing five zinc finger domains. It has been implicated as a mediator of the Sonic hedgehog signal in chicken limb development and is widely expressed in mesoderrn and ectoderm during mouse development. This evidence suggests GLI may function in multiple developmental programs including regulation of lestis-specific genes expressed at the onset of sperrnatogenesis. Supported by NIH grant #HD05863.
|Original language||English (US)|
|State||Published - Dec 1 1997|
ASJC Scopus subject areas
- Molecular Biology