Glial alterations in the hippocampus of rats submitted to ibotenic-induced lesion of the nucleus basalis magnocellularis

Alessandra Swarowsky, Letícia Rodrigues, Regina Biasibetti, Marina C. Leite, Lucas Fürstenau de Oliveira, Lucia M V de Almeida, Carmem Gottfried, Jorge A. Quillfeldt, Matilde Achaval, Carlos Alberto Gonçalves*

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Lesion of the nucleus basalis magnocellularis (nbm) is a suitable approach to study cognitive deficit and behavior alterations involving cholinergic dysfunction, which is associated with the major types of dementia. Cortical astrogliosis also has been described in this model, but it is not clear whether hippocampal astrocytes are activated. In this study, we investigated possible specific astrocyte alterations in the hippocampi of Wistar rats submitted to nbm damage with ibotenic acid, investigating the content and immunohistochemistry of glial fibrillary acidic protein (GFAP), as well as S100B protein content, glutamate uptake and glutamine synthetase activity on the 7th and 28th post-lesion days. Cognitive deficit was confirmed by the step-down inhibitory avoidance task. Interestingly, we found a decrease in GFAP content, S100B content and glutamate uptake activity in the hippocampus on the 28th day after nbm lesion. No alterations were observed in glutamine synthetase activity or in the cerebrospinal fluid S100B content. Although our data suggest caution in the use of nbm lesion with ibotenic acid as a dementia model, it is possible that these alterations could contribute to the cognitive deficit observed in these rats.

Original languageEnglish (US)
Pages (from-to)206-211
Number of pages6
JournalBehavioural Brain Research
Volume190
Issue number2
DOIs
StatePublished - Jul 19 2008

Keywords

  • GFAP
  • Glutamate uptake
  • Hippocampus
  • Ibotenic acid
  • Nbm lesion
  • S100B

ASJC Scopus subject areas

  • Behavioral Neuroscience

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    Swarowsky, A., Rodrigues, L., Biasibetti, R., Leite, M. C., de Oliveira, L. F., de Almeida, L. M. V., Gottfried, C., Quillfeldt, J. A., Achaval, M., & Gonçalves, C. A. (2008). Glial alterations in the hippocampus of rats submitted to ibotenic-induced lesion of the nucleus basalis magnocellularis. Behavioural Brain Research, 190(2), 206-211. https://doi.org/10.1016/j.bbr.2008.02.039