@article{4ec029d0bc9d4fa9bd8ead866b2fc800,
title = "Global and Regional Damages in Retinal Ganglion Cell Axon Bundles Monitored Non-Invasively by Visible-Light Optical Coherence Tomography Fibergraphy",
abstract = "Retinal ganglion cells (RGCs) exhibit compartmentalized organization, receiving synaptic inputs through their dendrites and transmitting visual information from the retina to the brain through the optic nerve. Little is known about the structure of RGC axon bundles extending from individual RGC somas to the optic nerve head (ONH) and how they respond to disease insults. We recently introduced visible-light optical coherence tomography fibergraphy (vis-OCTF), a technique for directly visualizing and analyzing mouse RGC axon bundles in vivo. In this study, we validated vis-OCTF{\textquoteright}s ability to quantify RGC axon bundles with an increased number of RGCs using mice deficient in BCL2-associated X protein (BAX-/-). Next, we performed optic nerve crush (ONC) injury on wild-type (WT) mice and showed that the changes in RGC axon bundle width and thickness were location-dependent. Our work demonstrates the potential of vis-OCTF to longitudinally quantify and track RGC damage at single axon bundle level in optic neuropathies.",
keywords = "axon bundles, in vivo imaging, optic neuropathy, retinal ganglion cell, vis-OCT fibergram, visible light optical coherence tomography",
author = "Marta Grannonico and Miller, {David A.} and Mingna Liu and Pedro Norat and Deppmann, {Christopher D.} and Netland, {Peter A.} and Zhang, {Hao F.} and Xiaorong Liu",
note = "Funding Information: Received Apr. 19, 2021; revised Aug. 18, 2021; accepted Oct. 15, 2021. Author contributions: X.L., M.G., D.A.M., M.L., and H.F.Z. designed research; X.L., M.G., D.A.M., M.L., P.N., and H.F.Z. performed research; X.L., C.D.D., and H.F.Z. contributed unpublished reagents/analytic tools; X.L., M.G., D.A.M., M.L., P.N., P.A.N., and H.F.Z. analyzed data; X.L., M.G., and D.A.M. wrote the first draft of the paper; X.L., M.G., D.A.M., M.L., P.N., C.D.D., P.A.N., and H.F.Z. edited the paper; X.L., M.G., D.A.M., and H.F.Z. wrote the paper. This work was supported in part by National Institutes of Health Grants R01EY026078, R01EY029121, R01EY028304, R01EY019949, R01EY026286, and R44EY026466. We thank Ashley Mason for her help with BAX mice breeding. We also thank Jingyi Gao, James Cole, and Prof. Ignacio Provencio for their fruitful discussions. *M.G., D.A.M., and M.L. contributed equally to this work. H.F.Z. has financial interests in Opticent Health, which did not support this work. All other authors declare no competing financial interests. Correspondence should be addressed to Hao F. Zhang at hfzhang@northwestern.edu or Xiaorong Liu at xl8n@virginia.edu. https://doi.org/10.1523/JNEUROSCI.0844-21.2021 Copyright {\textcopyright} 2021 the authors Publisher Copyright: Copyright {\textcopyright} 2021 the authors",
year = "2021",
month = dec,
day = "8",
doi = "10.1523/JNEUROSCI.0844-21.2021",
language = "English (US)",
volume = "41",
pages = "10179--10193",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "49",
}