Gluconate metabolism is required for virulence of the soft-rot pathogen pectobacterium carotovorum

Beth Mole, Sohrab Habibi, Jeffery L. Dangl, Sarah R. Grant

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Pectobacterium carotovorum is a ubiquitous soft rot pathogen thatuses global virulence regulators to coordinate pathogenesis in response to undefined environmental conditions. We characterize an operon in P. carotovorum required for gluconate metabolism and virulence. The operon contains four genes that are highly conserved among proteobacteria (initially annotated ygbJKLM), one of which was misassigned as a type III secreted effector, (ygbK, originally known as hopAN1). A mutant with a deletion-insertion within this operon is unable to metabolize gluconate, a precursor for the pentose phosphate pathway. The mutant exhibits attenuated growth on the leaves of its host of isolation, potato, and those of Arabidopsis thaliana. Notably, the mutant hypermacerates potato tubers and is deficient in motility. Global virulence regulators that are responsive to cell wall pectin breakdown products and other undefined environmental signals, KdgR and FlhD, respectively, are misregulated in the mutant. The alteration of virulence mediated via changes in transcription of known global virulence regulators in our ygbJ-M operon mutant suggests a role for host-derived catabolic intermediates in P. carotovorum pathogenesis. Thus, we rename this operon in P. carotovorum vguABCD for virulence and gluconate metabolism.

Original languageEnglish (US)
Pages (from-to)1335-1344
Number of pages10
JournalMolecular Plant-Microbe Interactions
Volume23
Issue number10
DOIs
StatePublished - Oct 2010

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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