Glitserinovye modeli mertsatel'nogo épiteliia slizistoǐ obolochki bronkhov i ikh ispol'zovanie dlia diagnostiki khronicheskikh nespetsificheskikh zabolevaniǐ legkikh.

N. A. Shanina; V. I. Gel'fand; I. I. Dykhanov; N. G. Bronza; N. N. Rozinova

Glitserinovye modeli mertsatel'nogo épiteliia slizistoǐ obolochki bronkhov i ikh ispol'zovanie dlia diagnostiki khronicheskikh nespetsificheskikh zabolevaniǐ legkikh.

Translated title of the contribution: Glycerol models of the ciliated epithelium of bronchial mucosa and their use in the diagnosis of chronic nonspecific lung diseases

N. A. Shanina^*, V. I. Gel'fand, I. I. Dykhanov, N. G. Bronza, N. N. Rozinova

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The method of extraction of ciliated epithelium from biopsy samples of human bronchial mucosa with glycerol is suggested. Permeabilized cilia of glycerol-extracted cells can be easily reactivated by exogenous ATP. This method was used for the study of ciliary dyskinesia in patients with chronic lung diseases. It was shown that in patients with Kartagener's syndrome neither freshly-isolated, nor glycerol-extracted ATP-treated cilia were motile. On the other hand, in some patients with bronchial asthma ATP reactivated glycerol-extracted cilia, while cilia of freshly-isolated cells remained immotile. The study shows that glycerol permeabilization and reactivation by ATP can be used for the analysis of cilial contractile apparatus in patients with chronic lung disease.

Translated title of the contribution	Glycerol models of the ciliated epithelium of bronchial mucosa and their use in the diagnosis of chronic nonspecific lung diseases
Original language	Russian
Pages (from-to)	84-86
Number of pages	3
Journal	Biulleten" eksperimental"noi biologii i meditsiny
Volume	101
Issue number	1
State	Published - Jan 1986

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Glitserinovye modeli mertsatel'nogo {\'e}piteliia slizistoǐ obolochki bronkhov i ikh ispol'zovanie dlia diagnostiki khronicheskikh nespetsificheskikh zabolevaniǐ legkikh.",

abstract = "The method of extraction of ciliated epithelium from biopsy samples of human bronchial mucosa with glycerol is suggested. Permeabilized cilia of glycerol-extracted cells can be easily reactivated by exogenous ATP. This method was used for the study of ciliary dyskinesia in patients with chronic lung diseases. It was shown that in patients with Kartagener's syndrome neither freshly-isolated, nor glycerol-extracted ATP-treated cilia were motile. On the other hand, in some patients with bronchial asthma ATP reactivated glycerol-extracted cilia, while cilia of freshly-isolated cells remained immotile. The study shows that glycerol permeabilization and reactivation by ATP can be used for the analysis of cilial contractile apparatus in patients with chronic lung disease.",

author = "Shanina, {N. A.} and Gel'fand, {V. I.} and Dykhanov, {I. I.} and Bronza, {N. G.} and Rozinova, {N. N.}",

year = "1986",

month = jan,

language = "Russian",

volume = "101",

pages = "84--86",

journal = "Biulleten{"} eksperimental{"}noi biologii i meditsiny",

issn = "0365-9615",

publisher = "Rossiiskaya Akademiya Meditsinskikh Nauk",

number = "1",

}

Glitserinovye modeli mertsatel'nogo épiteliia slizistoǐ obolochki bronkhov i ikh ispol'zovanie dlia diagnostiki khronicheskikh nespetsificheskikh zabolevaniǐ legkikh. / Shanina, N. A.; Gel'fand, V. I.; Dykhanov, I. I. et al.
In: Biulleten" eksperimental"noi biologii i meditsiny, Vol. 101, No. 1, 01.1986, p. 84-86.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Glitserinovye modeli mertsatel'nogo épiteliia slizistoǐ obolochki bronkhov i ikh ispol'zovanie dlia diagnostiki khronicheskikh nespetsificheskikh zabolevaniǐ legkikh.

AU - Shanina, N. A.

AU - Gel'fand, V. I.

AU - Dykhanov, I. I.

AU - Bronza, N. G.

AU - Rozinova, N. N.

PY - 1986/1

Y1 - 1986/1

N2 - The method of extraction of ciliated epithelium from biopsy samples of human bronchial mucosa with glycerol is suggested. Permeabilized cilia of glycerol-extracted cells can be easily reactivated by exogenous ATP. This method was used for the study of ciliary dyskinesia in patients with chronic lung diseases. It was shown that in patients with Kartagener's syndrome neither freshly-isolated, nor glycerol-extracted ATP-treated cilia were motile. On the other hand, in some patients with bronchial asthma ATP reactivated glycerol-extracted cilia, while cilia of freshly-isolated cells remained immotile. The study shows that glycerol permeabilization and reactivation by ATP can be used for the analysis of cilial contractile apparatus in patients with chronic lung disease.

AB - The method of extraction of ciliated epithelium from biopsy samples of human bronchial mucosa with glycerol is suggested. Permeabilized cilia of glycerol-extracted cells can be easily reactivated by exogenous ATP. This method was used for the study of ciliary dyskinesia in patients with chronic lung diseases. It was shown that in patients with Kartagener's syndrome neither freshly-isolated, nor glycerol-extracted ATP-treated cilia were motile. On the other hand, in some patients with bronchial asthma ATP reactivated glycerol-extracted cilia, while cilia of freshly-isolated cells remained immotile. The study shows that glycerol permeabilization and reactivation by ATP can be used for the analysis of cilial contractile apparatus in patients with chronic lung disease.

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SN - 0365-9615

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Glitserinovye modeli mertsatel'nogo épiteliia slizistoǐ obolochki bronkhov i ikh ispol'zovanie dlia diagnostiki khronicheskikh nespetsificheskikh zabolevaniǐ legkikh.

Abstract

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UN SDGs

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