Glycoconjugates of the tectorial membrane

Zhila Khalkhali-Ellis, Frank W. Hemming, Karen P. Steel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

The type and quantity of carbohydrate present in the tectorial membrane (TM) was analysed using gas-liquid chromatography and lectin staining of TM protein subunits previously separated by electrophoresis. A relatively large amount of carbohydrate was found, and glucose, N-acetylglucosamine, N-acetylgalactosamine, galactose, mannose and N-acetylneuraminic acid were detected. The presence of mannose and the reaction of many of the protein bands with lectins suggest that at least part of the carbohydrate present is in the form of glycoprotein. The reaction of the main protein band with the lectins RCA1 and ConA is consistent with the suggestion [Thalmann et al. (1985) J. Acoust. Soc. Am. Suppl. 1, Vol. 78, S66] that this band is similar to collagen type II. The failure to detect any uronic acid in these experiments indicates that the more common proteoglycans are probably not a major component of the TM (although keratan sulphate might be present).

Original languageEnglish (US)
Pages (from-to)185-191
Number of pages7
JournalHearing research
Volume25
Issue number2-3
DOIs
StatePublished - 1987

Keywords

  • gas-liquid chromatography
  • glycoconjugate
  • lectin
  • tectorial membrane

ASJC Scopus subject areas

  • Sensory Systems

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