Glycopolymer Microarrays with Sub-Femtomolar Avidity for Glycan Binding Proteins Prepared by Grafted-To/Grafted-From Photopolymerizations

Daniel J. Valles, Yerzhan S. Zholdassov, Joanna Korpanty, Samiha Uddin, Yasir Naeem, David R. Mootoo, Nathan C. Gianneschi, Adam B. Braunschweig*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We report a novel glycan array architecture that binds the mannose-specific glycan binding protein, concanavalin A (ConA), with sub-femtomolar avidity. A new radical photopolymerization developed specifically for this application combines the grafted-from thiol–(meth)acrylate polymerization with thiol–ene chemistry to graft glycans to the growing polymer brushes. The propagation of the brushes was studied by carrying out this grafted-to/grafted-from radical photopolymerization (GTGFRP) at >400 different conditions using hypersurface photolithography, a printing strategy that substantially accelerates reaction discovery and optimization on surfaces. The effect of brush height and the grafting density of mannosides on the binding of ConA to the brushes was studied systematically, and we found that multivalent and cooperative binding account for the unprecedented sensitivity of the GTGFRP brushes. This study further demonstrates the ease with which new chemistry can be tailored for an application as a result of the advantages of hypersurface photolithography.

Original languageEnglish (US)
Pages (from-to)20350-20357
Number of pages8
JournalAngewandte Chemie - International Edition
Volume60
Issue number37
DOIs
StatePublished - Sep 6 2021

Keywords

  • glycopolymers
  • lectins
  • lithography
  • microarrays
  • thiol–ene

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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