Glycosidase activities in hog serum, optic nerve, and ocular tissues

Ljuba Stojiljković, Božana Alexander, Svjetlana Stoisavljević-Šatara, Ranko Škrbić, Rajko Igić*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Using spectrophotometric assay, we have studied the distribution of α-D-mannosidase, β-D-galactosidase, and N-acetyl-β-D-glucosaminidase in hog serum, the aqueous humor, optic nerve, retina, and uvea (iris, ciliary body, choroid). N-acetyl-β-D-glucosaminidase was the most active gycosidase in all tissues that we studied. The highest activity of α-D-mannosidase was found in the extracts prepared from the iris, while β-D-galactosidase and N-acetyl-β-D-glucosaminidase were the most active in extracts of the choroid and ciliary body, respectively. The aqueous humor and serum had several times lower specific activity of glycosidases than did the extracts ofocular tissues or optic There was no significant difference between the activity of these enzymes in the aqueous humor and serum. High activity of glycosidases in the optic nerve suggests their role for intrinsic axonal repair. Retinal extracts had the smallest glycosidase activity of all ocular extracts prepared. The physiological role of these findings is discussed.

Original languageEnglish (US)
Pages (from-to)101-103
Number of pages3
JournalBiomedical Research
Volume25
Issue number2
DOIs
StatePublished - Apr 2004

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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