Abstract
Oxidative stress is well accepted as an important pathogenic factor in Parkinson disease, based largely on indirect evidence. Recently, we have developed antibodies that recognize specific advanced glycation end-products (anti-pentosidine and anti-pyrraline), protein modifications that are potentiated by oxidative stress in a process termed glycoxidation. We applied these antibodies immunocytochemically to affected regions in Parkinson disease and diffuse Lewy body disease brains. Additionally, we used antibodies to heme oxygenase-1, a putative marker of oxidative stress response. Immunoreactivity to pentosidine, pyrraline, and heme oxygenase-1 was seen in the substantia nigra of Parkinson disease and the neocortex of diffuse Lewy body disease. Heme oxygenase-1 was further demonstrated by immunoelectron microscopy in intimate association with filaments of cortical Lewy bodies. Immunolocalization of advanced glycation end-products and a marker of oxidative stress response induction provides evidence that glycoxidation and oxidative stress may be an important pathogenic factor in diseases characterized by Lewy body formation, and furthers the evidence that cytoskeletal proteins and their inclusions are susceptible to oxidative stress.
Original language | English (US) |
---|---|
Pages (from-to) | 195-200 |
Number of pages | 6 |
Journal | Brain research |
Volume | 737 |
Issue number | 1-2 |
DOIs | |
State | Published - Oct 21 1996 |
Funding
Work in the authors' laboratories is supported by grants from the National Institutes of Health, the American Health Assistance Foundation, and the American Federation of Aging Research. M.A.S. is a fellow of the American Philosophical Society. Gratitute is expressed to Vincent M. Monnier who supplied antibodies to pyrraline and pentosidine.
Keywords
- Advanced glycation end-product
- Diffuse Lewy body disease
- Immunocytochemistry
- Oxidative stress
- Parkinson's disease
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology