Glypican 3 has a higher sensitivity than alpha-fetoprotein for testicular and ovarian yolk sac tumour: Immunohistochemical investigation with analysis of histological growth patterns

Debra L. Zynger, John C. McCallum, Chunyan Luan, Pauline M. Chou, Ximing J. Yang

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Aims: Glypican 3 (GPC3) has been reported to be overexpressed in yolk sac tumour (YST), but the sensitivity has not been compared with β-fetoprotein (AFP). YST can form numerous growth patterns and the expression of GPC3 in these patterns has not been studied. The aim was to address these aspects. Methods and results: Sections from testicular or ovarian YST were subjected to immunohistochemistry using GPC3 (n = 39) and AFP (n = 24). Overall immunoreactivity for each case and specific histological patterns were semiquantitatively evaluated (0-3+) and intensity of reactivity was scored (0-3). All cases expressed GPC3 (1+, 5%; 2+, 8%; 3+, 87%) with strong intensity (2.9). The majority expressed AFP (58%) but immunoreactivity was often focal (0, 42%; 1+, 33%; 2+, 25%) and intensity was low (1.0). Using GPC3, >75% of the microcystic (n = 38), macrocystic (n = 26), solid (n = 21), glandular-alveolar (n = 8), endodermal sinus (n = 7), polyvesicular vitelline (n = 5), enteric (n = 4) and micropapillary (n = 2) growth patterns displayed 2+ or 3+ positivity. Conclusions: YST can display a variety of growth patterns that can be confused with other germ cell tumour components. GPC3 detects all growth patterns tested and has a higher sensitivity for detecting YST than AFP.

Original languageEnglish (US)
Pages (from-to)750-757
Number of pages8
JournalHistopathology
Volume56
Issue number6
DOIs
StatePublished - May 2010

Keywords

  • Glypican 3
  • Immunohistochemistry
  • Yolk sac tumour
  • β-fetoprotein

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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