Gobichelin A and B: Mixed-ligand siderophores discovered using proteomics

Yunqiu Chen; Michelle Unger; Ioanna Ntai; Ryan A. McClure; Jessica C. Albright; Regan J. Thomson; Neil L. Kelleher

doi:10.1039/c2md20232h

Gobichelin A and B: Mixed-ligand siderophores discovered using proteomics

Yunqiu Chen, Michelle Unger, Ioanna Ntai, Ryan A. McClure, Jessica C. Albright, Regan J. Thomson, Neil L. Kelleher^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

"Omic" strategies have been increasingly applied to natural product discovery processes, with (meta-)genome sequencing and mining implemented in many laboratories to date. Using the proteomics-based discovery platform called PrISM (Proteomic Investigation of Secondary Metabolism), we discovered two new siderophores gobichelin A and B from Streptomyces sp. NRRL F-4415, a strain without a sequenced genome. Using the proteomics information as a guide, the 37 kb gene cluster responsible for production of gobichelins was sequenced and its 20 open reading frames interpreted into a biosynthetic scheme. This led to the targeted detection and structure elucidation of the new compounds produced by nonribosomal peptide (NRP) synthesis.

Original language	English (US)
Pages (from-to)	233-238
Number of pages	6
Journal	MedChemComm
Volume	4
Issue number	1
DOIs	https://doi.org/10.1039/c2md20232h
State	Published - Jan 2013

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Organic Chemistry

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AU - Ntai, Ioanna

AU - McClure, Ryan A.

AU - Albright, Jessica C.

AU - Thomson, Regan J.

AU - Kelleher, Neil L.

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Gobichelin A and B: Mixed-ligand siderophores discovered using proteomics

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