Gobichelin A and B: Mixed-ligand siderophores discovered using proteomics

Yunqiu Chen, Michelle Unger, Ioanna Ntai, Ryan A. McClure, Jessica C. Albright, Regan J. Thomson, Neil L. Kelleher*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

"Omic" strategies have been increasingly applied to natural product discovery processes, with (meta-)genome sequencing and mining implemented in many laboratories to date. Using the proteomics-based discovery platform called PrISM (Proteomic Investigation of Secondary Metabolism), we discovered two new siderophores gobichelin A and B from Streptomyces sp. NRRL F-4415, a strain without a sequenced genome. Using the proteomics information as a guide, the 37 kb gene cluster responsible for production of gobichelins was sequenced and its 20 open reading frames interpreted into a biosynthetic scheme. This led to the targeted detection and structure elucidation of the new compounds produced by nonribosomal peptide (NRP) synthesis.

Original languageEnglish (US)
Pages (from-to)233-238
Number of pages6
JournalMedChemComm
Volume4
Issue number1
DOIs
StatePublished - Jan 2013

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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