Goblet cell degranulation in isolated canine tracheal epithelium: Response to exogenous ATP, ADP, and adenosine

C. W. Davis*, M. L. Dowell, M. Lethem, M. Van Scott

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Mucin secretion by goblet cells was determined by quantifying degranulation events (DE) in isolated, superficial epithelium from canine trachea. The epithelium was isolated and explanted to a novel transparent, permeable support, and the goblet cells were visualized by video microscopy. Baseline degranulation events were quantified at 0.05 DE/min. Luminal ATP (10-4 M, n = 10) stimulated a biphasic secretory response; a burst, maximum rate = 87.9 ± 25.3, was followed by a plateau, rate = 1.9 ± 0.3 DE/min. Serosal ATP elicited a complex set of responses: 9 cells failed to respond, 13 exhibited a trivial response, and 31 responded vigorously but with highly variable patterns of degranulation. Nonhydrolyzable 5'- adenylylimidodiphosphate caused degranulation from both sides of the epithelium. Luminal ADP and adenosine were ineffective. Serosal ADP and adenosine elicited a range of responses that was similar in diversity and magnitude to the ATP response. Our conclusions were as follows: 1) goblet cells in the superficial epithelium of the airway can be studied at the single-cell level in explants; 2) nucleotides stimulate goblet cells to secrete mucin; and 3) the goblet cell expresses different nucleotide receptors on its apical and basolateral membranes.

Original languageEnglish (US)
Pages (from-to)C1313-C1323
JournalAmerican Journal of Physiology - Cell Physiology
Volume262
Issue number5 31-5
DOIs
StatePublished - 1992
Externally publishedYes

Keywords

  • cell culture
  • exocytosis
  • mucin
  • nucleotide
  • purinergic
  • purinoceptor
  • secretion
  • video microscopy

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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