Graphene-induced adsorptive and optical artifacts during in vitro toxicology assays

Megan A. Creighton; J. Rene Rangel-Mendez; Jiaxing Huang; Agnes B. Kane; Robert H. Hurt

doi:10.1002/smll.201202625

Graphene-induced adsorptive and optical artifacts during in vitro toxicology assays

Megan A. Creighton, J. Rene Rangel-Mendez, Jiaxing Huang, Agnes B. Kane, Robert H. Hurt^*

^*Corresponding author for this work

Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the interaction, and/or (3) absorbing or scattering light, which are issues for both colorimetric and fluorescent assays.

Original language	English (US)
Pages (from-to)	1921-1927
Number of pages	7
Journal	Small
Volume	9
Issue number	11
DOIs	https://doi.org/10.1002/smll.201202625
State	Published - Jun 10 2013

Funding

Keywords

adsorption
fluorescence
graphene
micronutrients
toxicology

ASJC Scopus subject areas

General Chemistry
Engineering (miscellaneous)
Biotechnology
General Materials Science
Biomaterials

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10.1002/smll.201202625

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title = "Graphene-induced adsorptive and optical artifacts during in vitro toxicology assays",

abstract = "Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the interaction, and/or (3) absorbing or scattering light, which are issues for both colorimetric and fluorescent assays.",

keywords = "adsorption, fluorescence, graphene, micronutrients, toxicology",

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AU - Creighton, Megan A.

AU - Rangel-Mendez, J. Rene

AU - Huang, Jiaxing

AU - Kane, Agnes B.

AU - Hurt, Robert H.

PY - 2013/6/10

Y1 - 2013/6/10

N2 - Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the interaction, and/or (3) absorbing or scattering light, which are issues for both colorimetric and fluorescent assays.

AB - Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the interaction, and/or (3) absorbing or scattering light, which are issues for both colorimetric and fluorescent assays.

KW - adsorption

KW - fluorescence

KW - graphene

KW - micronutrients

KW - toxicology

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ER -

Graphene-induced adsorptive and optical artifacts during in vitro toxicology assays

Abstract

Funding

Keywords

ASJC Scopus subject areas

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