Abstract
Background: Chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) is characterized by type 2 inflammation with high levels of Th2 cytokines. Although T helper cytokines are released from T cells, innate lymphoid cells (ILC) are also known to produce high levels of the same cytokines.However, the presence of various types of ILC in CRS is poorly understood. Objective: The objective of this study was to fully characterize the presence of all ILC subsets in CRS and to identify phenotypical differences of group 2 ILC (ILC2) in CRSwNP compared to ILC2 from non-type 2 inflamed areas. Methods: We investigated the presence of ILC subsets in peripheral blood mononuclear cells (PBMC) from healthy subjects, tonsil tissue, ethmoid tissue from control subjects and patients with non-polypoid CRS (CRSsNP) and CRSwNP, as well as nasal polyp (NP) tissue from CRSwNP by flow cytometry. Sorted ILC2 were cultured in the presence and absence of IL-33 and production of IL-5 and IL-13 was assessed by Luminex. Results: We found that all ILC subsets were present in NP but ILC2 were dominant and significantly elevated compared to PBMC, tonsil, CRSsNP, and normal sinus tissue. We also found that inducible T-cell co-stimulator (ICOS) and side scatter were increased and CD127 was down-regulated in ILC2 from NP compared to blood or tonsil ILC2. Thymic stromal lymphopoietin, IL-7, and IL-33 were able to down-regulate expression of CD127 and increase side scatter in blood ILC2. Furthermore, sorted NP ILC2 but not blood ILC2 spontaneously released type 2 cytokines including IL-5 and IL-13. Conclusions and Clinical Relevance: These results suggest that ILC2 are not only elevated but also activated in CRSwNP in vivo and that ILC2 may play important roles in the type 2 inflammation in CRSwNP.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 233-243 |
| Number of pages | 11 |
| Journal | Immunity Inflammation and Disease |
| Volume | 5 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 2017 |
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Keywords
- Chronic rhinosinusitis
- ILC2
- Innate lymphoid cells
- Nasal polyp
- Type 2 inflammation
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
}
Group 2 innate lymphoid cells are elevated and activated in chronic rhinosinusitis with nasal polyps. / Poposki, Julie A.; Klingler, Aiko I.; Tan, Bruce Kuang-Huay; Soroosh, Pejman; Banie, Homayon; Lewis, Gavin; Hulse, Kathryn E; Stevens, Whitney; Peters, Anju Tripathi; Grammer III, Leslie C; Schleimer, Robert P; Welch, Kevin Christian; Smith, Stephanie Shintani; Conley Jr, David B; Raviv, Joseph R.; Karras, James G.; Akbari, Omid; Kern, Robert C; Kato, Atsushi.
In: Immunity Inflammation and Disease, Vol. 5, No. 3, 01.01.2017, p. 233-243.Research output: Contribution to journal › Article
TY - JOUR
T1 - Group 2 innate lymphoid cells are elevated and activated in chronic rhinosinusitis with nasal polyps
AU - Poposki, Julie A.
AU - Klingler, Aiko I.
AU - Tan, Bruce Kuang-Huay
AU - Soroosh, Pejman
AU - Banie, Homayon
AU - Lewis, Gavin
AU - Hulse, Kathryn E
AU - Stevens, Whitney
AU - Peters, Anju Tripathi
AU - Grammer III, Leslie C
AU - Schleimer, Robert P
AU - Welch, Kevin Christian
AU - Smith, Stephanie Shintani
AU - Conley Jr, David B
AU - Raviv, Joseph R.
AU - Karras, James G.
AU - Akbari, Omid
AU - Kern, Robert C
AU - Kato, Atsushi
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Background: Chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) is characterized by type 2 inflammation with high levels of Th2 cytokines. Although T helper cytokines are released from T cells, innate lymphoid cells (ILC) are also known to produce high levels of the same cytokines.However, the presence of various types of ILC in CRS is poorly understood. Objective: The objective of this study was to fully characterize the presence of all ILC subsets in CRS and to identify phenotypical differences of group 2 ILC (ILC2) in CRSwNP compared to ILC2 from non-type 2 inflamed areas. Methods: We investigated the presence of ILC subsets in peripheral blood mononuclear cells (PBMC) from healthy subjects, tonsil tissue, ethmoid tissue from control subjects and patients with non-polypoid CRS (CRSsNP) and CRSwNP, as well as nasal polyp (NP) tissue from CRSwNP by flow cytometry. Sorted ILC2 were cultured in the presence and absence of IL-33 and production of IL-5 and IL-13 was assessed by Luminex. Results: We found that all ILC subsets were present in NP but ILC2 were dominant and significantly elevated compared to PBMC, tonsil, CRSsNP, and normal sinus tissue. We also found that inducible T-cell co-stimulator (ICOS) and side scatter were increased and CD127 was down-regulated in ILC2 from NP compared to blood or tonsil ILC2. Thymic stromal lymphopoietin, IL-7, and IL-33 were able to down-regulate expression of CD127 and increase side scatter in blood ILC2. Furthermore, sorted NP ILC2 but not blood ILC2 spontaneously released type 2 cytokines including IL-5 and IL-13. Conclusions and Clinical Relevance: These results suggest that ILC2 are not only elevated but also activated in CRSwNP in vivo and that ILC2 may play important roles in the type 2 inflammation in CRSwNP.
AB - Background: Chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) is characterized by type 2 inflammation with high levels of Th2 cytokines. Although T helper cytokines are released from T cells, innate lymphoid cells (ILC) are also known to produce high levels of the same cytokines.However, the presence of various types of ILC in CRS is poorly understood. Objective: The objective of this study was to fully characterize the presence of all ILC subsets in CRS and to identify phenotypical differences of group 2 ILC (ILC2) in CRSwNP compared to ILC2 from non-type 2 inflamed areas. Methods: We investigated the presence of ILC subsets in peripheral blood mononuclear cells (PBMC) from healthy subjects, tonsil tissue, ethmoid tissue from control subjects and patients with non-polypoid CRS (CRSsNP) and CRSwNP, as well as nasal polyp (NP) tissue from CRSwNP by flow cytometry. Sorted ILC2 were cultured in the presence and absence of IL-33 and production of IL-5 and IL-13 was assessed by Luminex. Results: We found that all ILC subsets were present in NP but ILC2 were dominant and significantly elevated compared to PBMC, tonsil, CRSsNP, and normal sinus tissue. We also found that inducible T-cell co-stimulator (ICOS) and side scatter were increased and CD127 was down-regulated in ILC2 from NP compared to blood or tonsil ILC2. Thymic stromal lymphopoietin, IL-7, and IL-33 were able to down-regulate expression of CD127 and increase side scatter in blood ILC2. Furthermore, sorted NP ILC2 but not blood ILC2 spontaneously released type 2 cytokines including IL-5 and IL-13. Conclusions and Clinical Relevance: These results suggest that ILC2 are not only elevated but also activated in CRSwNP in vivo and that ILC2 may play important roles in the type 2 inflammation in CRSwNP.
KW - Chronic rhinosinusitis
KW - ILC2
KW - Innate lymphoid cells
KW - Nasal polyp
KW - Type 2 inflammation
UR - http://www.scopus.com/inward/record.url?scp=85029592980&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029592980&partnerID=8YFLogxK
U2 - 10.1002/iid3.161
DO - 10.1002/iid3.161
M3 - Article
VL - 5
SP - 233
EP - 243
JO - Immunity, inflammation and disease
JF - Immunity, inflammation and disease
SN - 2050-4527
IS - 3
ER -