Growth factors can protect b-chronic lymphocytic leukaemia cells against programmed cell death without stimulating proliferation

A. P. Jewell*, P. M. Lydyard, C. P. Worman, F. J. Giles, A. H. Goldstone

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The proliferation and survival of B-chronic lymphocytic leukaemia (B-CLL) cells may be regulated by autocrine growth factor loops. Furthermore, it has been suggested the reduction in lymphocytosis following therapy with interferon-alpha may be associated with the interruption of autocrine growth factor production. We have therefore examined the effects of a number of cytokines on the proliferation of B-CLL cells, and also on the regulation of programmed cell death, and the role of interferon-alpha in these systems. In the ten patients studied, neither interferon-alpha alone or together with either interferon-gamma, IL1, IL4, IL6, TNF, or serum containing high levels of soluble CD23 was able to induce proliferation of B-CLL cells. Incubation with TPA or IL2 resulted in variable proliferative responses. Co-incubation with interferon-alpha enhanced TPA-induced proliferation in 4 cases, but reduced IL2-induced proliferation in all cases studied. In contrast, all the cytokines studied were able to protect B-CLL cells against programmed cell death, both spontaneous and that induced by hydrocortisone, with the exception of TNF. These data suggest a role for interferon-alpha in disrupting autocrine survival pathways rather than inhibiting proliferation.

Original languageEnglish (US)
Pages (from-to)159-162
Number of pages4
JournalLeukemia and Lymphoma
Volume18
Issue number1-2
DOIs
StatePublished - Jan 1 1995

Keywords

  • Apoptosis
  • CLL
  • Cytokines

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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