Growth, morphologic, and invasive characteristics of early and late passages of a human endometrial carcinoma cell line (RL95-2)

Padma Sundareshan*, Mary J.C. Hendrix

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Two in vitro passages of a human endometrial adenocarcinoma continuous cell line (RL95-2), an early (subcultured <30 times) and a late passage (subcultured >200 times) have provided an interesting model to study the growth, morphologic, and invasive properties of endometrial tumors. The early passage, which has been shown to be estrogen-receptor positive, has characteristics closely resembling a primary tumor, whereas the estrogen receptor negative late passage exhibits several features of the metastatic phenotype. Compared to the early passage cells, the late passage cells were less serum dependent, formed foci, demonstrated a faster rate of growth (due to their shorter doubling times), and attained higher saturation densities. The late passage cells also displayed an altered morphology which was accompanied by alterations in the distribution of F-actin. Even though early and late passages showed similar invasive potential in an in vitro invasion assay, the late passage cells, by virtue of their several transformed characteristics, maintain distinctive properties compared with their early passage counterparts.

Original languageEnglish (US)
Pages (from-to)544-552
Number of pages9
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume28
Issue number7-8
DOIs
StatePublished - Aug 1 1992

Keywords

  • endometrial adenocarcinoma
  • growth
  • invasion
  • morphology

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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