Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo

Michael P. Murphy*, Hülya Bayir, Vsevolod Belousov, Christopher J. Chang, Kelvin J.A. Davies, Michael J. Davies, Tobias P. Dick, Toren Finkel, Henry J. Forman, Yvonne Janssen-Heininger, David Gems, Valerian E. Kagan, Balaraman Kalyanaraman, Nils Göran Larsson, Ginger L. Milne, Thomas Nyström, Henrik E. Poulsen, Rafael Radi, Holly Van Remmen, Paul T. SchumackerPaul J. Thornalley, Shinya Toyokuni, Christine C. Winterbourn, Huiyong Yin, Barry Halliwell*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

419 Scopus citations


Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.

Original languageEnglish (US)
Pages (from-to)651-662
Number of pages12
JournalNature Metabolism
Issue number6
StatePublished - Jun 2022

ASJC Scopus subject areas

  • Physiology (medical)
  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Cell Biology


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