H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes

Eric G. Pamer; Chyung Ru Wang; Lorraine Flaherty; Kirsten Fischer Lindahl; Michael J. Bevan

doi:10.1016/0092-8674(92)90097-V

H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes

Eric G. Pamer^*, Chyung Ru Wang, Lorraine Flaherty, Kirsten Fischer Lindahl, Michael J. Bevan

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

192 Scopus citations

Abstract

We report evidence that a major histocompatibility complex-encoded nonclassic class I molecule presents a foreign peptide to cytotoxic T lymphocytes (CTL) during an infection. Mice immunized with virulent Listeria monocytogenes generate CD8⁺ CTL with αβ receptors specific for a bacterial peptide presented by a conserved class I molecule encoded in the M region of the major histocompatibility complex. The Listeria peptide is digested by carboxypeptidase Y but resists aminopeptidase M, and only peptides with N-formyl methionine competitively block its presentation to CTL. Transfection with the H-2M3^d gene enables a negative (H-2^w17) cell line to present the bacterial peptide. One function, therefore, of H-2M3 is to present bacterial peptides to CTL during infection.

Original language	English (US)
Pages (from-to)	215-223
Number of pages	9
Journal	Cell
Volume	70
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(92)90097-V
State	Published - Jul 24 1992

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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@article{7087d7fbda564c21bd1f05ad2e205936,

title = "H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes",

abstract = "We report evidence that a major histocompatibility complex-encoded nonclassic class I molecule presents a foreign peptide to cytotoxic T lymphocytes (CTL) during an infection. Mice immunized with virulent Listeria monocytogenes generate CD8+ CTL with αβ receptors specific for a bacterial peptide presented by a conserved class I molecule encoded in the M region of the major histocompatibility complex. The Listeria peptide is digested by carboxypeptidase Y but resists aminopeptidase M, and only peptides with N-formyl methionine competitively block its presentation to CTL. Transfection with the H-2M3d gene enables a negative (H-2w17) cell line to present the bacterial peptide. One function, therefore, of H-2M3 is to present bacterial peptides to CTL during infection.",

author = "Pamer, {Eric G.} and Wang, {Chyung Ru} and Lorraine Flaherty and Lindahl, {Kirsten Fischer} and Bevan, {Michael J.}",

note = "Funding Information: This work was supported by the National Institute of Allergy and Infectious Diseases and the Howard Hughes Medical Institute. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1992",

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T1 - H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes

AU - Pamer, Eric G.

AU - Wang, Chyung Ru

AU - Flaherty, Lorraine

AU - Lindahl, Kirsten Fischer

AU - Bevan, Michael J.

N1 - Funding Information: This work was supported by the National Institute of Allergy and Infectious Diseases and the Howard Hughes Medical Institute. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1992/7/24

Y1 - 1992/7/24

N2 - We report evidence that a major histocompatibility complex-encoded nonclassic class I molecule presents a foreign peptide to cytotoxic T lymphocytes (CTL) during an infection. Mice immunized with virulent Listeria monocytogenes generate CD8+ CTL with αβ receptors specific for a bacterial peptide presented by a conserved class I molecule encoded in the M region of the major histocompatibility complex. The Listeria peptide is digested by carboxypeptidase Y but resists aminopeptidase M, and only peptides with N-formyl methionine competitively block its presentation to CTL. Transfection with the H-2M3d gene enables a negative (H-2w17) cell line to present the bacterial peptide. One function, therefore, of H-2M3 is to present bacterial peptides to CTL during infection.

AB - We report evidence that a major histocompatibility complex-encoded nonclassic class I molecule presents a foreign peptide to cytotoxic T lymphocytes (CTL) during an infection. Mice immunized with virulent Listeria monocytogenes generate CD8+ CTL with αβ receptors specific for a bacterial peptide presented by a conserved class I molecule encoded in the M region of the major histocompatibility complex. The Listeria peptide is digested by carboxypeptidase Y but resists aminopeptidase M, and only peptides with N-formyl methionine competitively block its presentation to CTL. Transfection with the H-2M3d gene enables a negative (H-2w17) cell line to present the bacterial peptide. One function, therefore, of H-2M3 is to present bacterial peptides to CTL during infection.

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H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes

Abstract

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