Hair loss and defective T- and B-cell function in mice lacking ORAI1

Yousang Gwack, Sonal Srikanth, Masatsugu Oh-hora, Patrick G. Hogan, Edward D. Lamperti, Megumi Yamashita, Curtis Gelinas, Daniel S. Neems, Yoshiteru Sasaki, Stefan Feske, Murali Prakriya, Klaus Rajewsky, Anjana Rao*

*Corresponding author for this work

Research output: Contribution to journalArticle

217 Scopus citations

Abstract

ORAI1 is a pore subunit of the store-operated Ca2+ release-activated Ca2+ (CRAC) channel. To examine the physiological consequences of ORAI1 deficiency, we generated mice with targeted disruption of the Orai1 gene. The results of immunohistochemical analysis showed that ORAI1 is expressed in lymphocytes, skin, and muscle of wild-type mice and is not expressed in Orai1-/- mice. Orai1-/- mice with the inbred C57BL/6 background showed perinatal lethality, which was overcome by crossing them to outbred ICR mice. Orai1-/- mice were small in size, with eyelid irritation and sporadic hair loss resembling the cyclical alopecia observed in mice with keratinocyte-speciflc deletion of the Cnb1 gene. T and B cells developed normally in Orai1-/- mice, but B cells showed a substantial decrease in Ca2+ influx and cell proliferation in response to B-cell receptor stimulation. Naïve and differentiated Orai1-/- T cells showed substantial reductions in store-operated Ca2+ entry, CRAC currents, and cytokine production. These features are consistent with the severe combined immunodeficiency and mild extraimmunological symptoms observed in a patient with a missense mutation in human ORAI1 and distinguish the ORAI1-null mice described here from a previously reported Orai1 gene-trap mutant mouse which may be a hypomorph rather than a true null.

Original languageEnglish (US)
Pages (from-to)5209-5222
Number of pages14
JournalMolecular and cellular biology
Volume28
Issue number17
DOIs
StatePublished - Sep 1 2008

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Hair loss and defective T- and B-cell function in mice lacking ORAI1'. Together they form a unique fingerprint.

  • Cite this

    Gwack, Y., Srikanth, S., Oh-hora, M., Hogan, P. G., Lamperti, E. D., Yamashita, M., Gelinas, C., Neems, D. S., Sasaki, Y., Feske, S., Prakriya, M., Rajewsky, K., & Rao, A. (2008). Hair loss and defective T- and B-cell function in mice lacking ORAI1. Molecular and cellular biology, 28(17), 5209-5222. https://doi.org/10.1128/MCB.00360-08