Halothane potentiates the antitumor activity of gamma-interferon and mimics calmodulin-blocking agents

S. Rudnick, G. W. Stevenson, S. C. Hall, I. Espinoza-Delgado, H. C. Stevenson*, D. L. Longo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

This study reports effects of halothane on tumor cells in vitro. Cells from the human colon cancer cell line HT-29 were exposed to various concentrations of halothane for 8-72 h. The effect of this exposure on this colon cancer cell line, with and without coincubation with the biologic response modifier gamma-interferon (IFN-γ), was studied. Using the tumor target cell survival (TTCS) assay, concentrations of halothane from 0.5 to 2% markedly augmented the antitumor activities of IFN-γ against HT-29. The tumor cell cytostatic effects of IFN-γ in the 0.75-6-unit/ml range were increased nearly 400% by concentrations of halothane as low as 1%. These results were confirmed in a separate cytolytic assay (Indium-111 release assay), which revealed that halothane concentrations in the 2-4% range markedly increased the cytolytic capacity of IFN-γ at doses of IFN-γ between 75 and 1,250 units/ml. The cytolytic activity of IFN-γ was increased nearly 300% by doses of halothane as low as 1%. A nearly identical pattern of augmentation of IFN-γ-induced antitumor activity was observed when the known calmodulin inhibitor trifluoperazine (TFP) was coincubated with IFN-γ. At concentrations of 4-10 μM, the antitumor activity of IFN-γ was increased nearly 400%. These observations suggest that the pattern of halothane potentiation of the antitumor activity of IFN-γ is similar to that exhibited by known calmodulin inhibitors.

Original languageEnglish (US)
Pages (from-to)115-119
Number of pages5
JournalAnesthesiology
Volume74
Issue number1
DOIs
StatePublished - Jan 1 1991

Keywords

  • anesthetics, volatile
  • calmodulin inhibitors
  • gamma-interferon
  • halothane
  • trifluoperazine (TFP)
  • tumor cell cytotoxicity

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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