Hammerhead redux: Does the new structure fit the old biochemical data?

Jennifer A. Nelson, Olke C. Uhlenbeck

Research output: Contribution to journalReview article

45 Scopus citations

Abstract

The cleavage rates of 78 hammerhead ribozymes containing structurally conservative chemical modifications were collected from the literature and compared to the recently determined crystal structure of the Schistosoma mansoni hammerhead. With only a few exceptions, the biochemical data were consistent with the structure, indicating that the new structure closely resembles the transition state of the reaction. Since all the biochemical data were collected on minimal hammerheads that have a very different structure, the minimal hammerhead must be dynamic and occasionally adopt the quite different extended structure in order to cleave. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)605-615
Number of pages11
JournalRNA
Volume14
Issue number4
DOIs
Publication statusPublished - Apr 1 2008

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Keywords

  • Catalytic core
  • RNA crystal structure
  • RNA dynamics
  • RNA structure-function
  • Ribozyme

ASJC Scopus subject areas

  • Molecular Biology

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