Hammerhead ribozymes with a faster cleavage rate

Beatrice Clouet-D'Orval, Olke C. Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

A hammerhead ribozyme that was previously reported to have a rate of chemical cleavage 10-fold faster than that of conventional hammerheads was analyzed in greater detail. Although originally found as a bimolecular hammerhead assembled through helices I and II, fast cleavage was observed in hammerheads in the more conventional helix I-helix III form, provided the sequence of helix of the fast hammerhead was preserved. Mutations indicated that the fast rate of cleavage was due to the presence of both the U1.1-A2.1 and A1.2-U2.2 base pairs. The faster rate of cleavage was due to a small increase in the activation entropy of the reaction. In addition, we confirmed previous reports that increasing the length of helix by greater than five base pairs inhibits cleavage slightly and have uncovered a similar effect in helix II.

Original languageEnglish (US)
Pages (from-to)9087-9092
Number of pages6
JournalBiochemistry
Volume36
Issue number30
DOIs
StatePublished - Jul 29 1997

ASJC Scopus subject areas

  • Biochemistry

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