Head-mounted optical imaging and optogenetic stimulation system for use in behaving primates

Derek Zaraza*, Mykyta M. Chernov, Yiyuan Yang, John A. Rogers, Anna W. Roe, Robert M. Friedman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Advances in optical technology have revolutionized studies of brain function in freely behaving mice. Here, we describe an optical imaging and stimulation device for use in primates that easily attaches to an intracranial chamber. It consists of affordable commercially available or 3D-printed components: a monochromatic camera, a small standard lens, a wireless μLED stimulator powered by an induction coil, and an LED array for illumination. We show that the intrinsic imaging performance of this device is comparable to a standard benchtop system in revealing the functional organization of the visual cortex for awake macaques in a primate chair or under anesthesia. Imaging revealed neural modulatory effects of wireless focal optogenetic stimulation aimed at identified functional domains. With a 1 to 2 cm field of view, 100× larger than previously used in primates without head restraint, our device permits widefield optical imaging and optogenetic stimulation for ethological studies in primates.

Original languageEnglish (US)
Article number100351
JournalCell Reports Methods
Issue number12
StatePublished - Dec 19 2022


  • imaging system
  • intrinsic optical imaging
  • macaque
  • mesoscale
  • optogenetic stimulation
  • visual cortex
  • widefield imaging

ASJC Scopus subject areas

  • Genetics
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Biotechnology
  • Computer Science Applications


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