Abstract
Formation of the atrioventricular channels and valves from the endocardial cushion occurs through growth and remodeling of the initial endocardial cushion. This process requires balanced coordination of proliferation and apoptosis by still unknown factors. To detect a possible role for the heat shock proteins 25 and 70 (HSP25 and HSP70) as apoptosis-associated proteins and differentiation factors in the development of the endocardial cushion, we analyzed their temporal and regional occurrence during cell proliferation and apoptosis in E11-E17 embryos. The distribution and timing of these events and factors were consistent with the hypothesis that HSP25 is related to myocardial development whereas HSP70 is related to differentiation of the endocardial cushion by cell proliferation and apoptosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-104 |
| Number of pages | 10 |
| Journal | Histochemistry and Cell Biology |
| Volume | 115 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2001 |
Funding
Acknowledgements We thank Mrs. Leena Salminen, Mrs. Sirpa From, and Mr. Urpo Reunanen for skillful technical assistance with the electron microscopy. This work was supported by the State grant for Helsinki University Central Hospital and by the Finnish Foundation for Cardiovascular Research.
Keywords
- Apoptosis
- Developing heart
- Endocardial cushion
- Heat shock proteins
- PCNA
ASJC Scopus subject areas
- Histology
- Molecular Biology
- Medical Laboratory Technology
- Cell Biology