TY - JOUR
T1 - Heat shockfactor 2-like activity in mouse blastocysts
AU - Mezger, Valérie
AU - Rallu, Murielle
AU - Morimoto, Richard I.
AU - Morange, Michel
AU - Renard, Jean Paul
PY - 1994/12
Y1 - 1994/12
N2 - The expression of heat shock genes is induced in all living cells by a series of proteotoxic treatments. Heat shock genes are also activated spontaneously during different phases of embryonic development. HSP89α and HSC70 are expressed at a high level in the mouse blastocyst. Afamily of factors, called HSFs, are able to bind to the promoters of heat shock genes on upstream conserved elements (HSEs). HSF1 is unable to bindto HSE sequences in absence of stress. It is activated after a stress by post-translational modifications and conformational change. HSF2 shows common structural domains with HSF1; however, it is active at normal temperatures. Recently we showedthe presence of an abundant HSE-binding activity in nonshocked blastocysts. We demonstrate here byusing polyclonal antibodies that HSF2 is the major constituent of this constitutive HSE-binding activity. HSF2 might be involved in the control of heat shock gene expression during early mammalianembryogenesis.
AB - The expression of heat shock genes is induced in all living cells by a series of proteotoxic treatments. Heat shock genes are also activated spontaneously during different phases of embryonic development. HSP89α and HSC70 are expressed at a high level in the mouse blastocyst. Afamily of factors, called HSFs, are able to bind to the promoters of heat shock genes on upstream conserved elements (HSEs). HSF1 is unable to bindto HSE sequences in absence of stress. It is activated after a stress by post-translational modifications and conformational change. HSF2 shows common structural domains with HSF1; however, it is active at normal temperatures. Recently we showedthe presence of an abundant HSE-binding activity in nonshocked blastocysts. We demonstrate here byusing polyclonal antibodies that HSF2 is the major constituent of this constitutive HSE-binding activity. HSF2 might be involved in the control of heat shock gene expression during early mammalianembryogenesis.
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U2 - 10.1006/dbio.1994.1361
DO - 10.1006/dbio.1994.1361
M3 - Article
C2 - 7813800
AN - SCOPUS:0028577405
SN - 0012-1606
VL - 166
SP - 819
EP - 822
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -