lnterferon-$gM (IFN-$gM) production by peripheral blood leukocytes from bladder cancer patients was compared with that of patients with prostate cancer and benign prostatic hyperplasia, non-tumor-bearing patients with bacterial infections, and normal controls. Leukocyte preparations including mononuclear cells isolated on a Ficoll-Hypaque density gradient (Fraction 2) and glass-nonadherent mononuclear cells (Fraction 3) were stimulated with Protein A from Staphylococcus aureus, and IFN-$gM production was monitored 24 hr late r. The class of interferon produced was identified by antibody neutralization experiments which clearly showed S. aureus Protein A-induced interferon to be IFN-$gM. There was significantly heightened IFN-$gM production by Fraction 3 cells from bladder cancer patients and patients with bacterial infections. Heightened IFN-$gM production by bladder cancer patients was not observed in the Fraction 2 cells. No correlation was observed between IFN-$gM production and patients with invasive or noninvasive bladder cancer, but IFN-$gM production was lower in patients having Stage C or D tumors than in those having Stage A or B tumors. These results in conjunction with previous reports demonstrating heightened IFN-$gM production during periods of antigenic stimulation suggest that bladder tumors may induce a cell-mediated immune response in the host as evidenced by the elevation in IFN-$gM production. Moreover, the results suggest that macrophages may be important regulators of IFN-$gM production in bladder cancer patients.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Jul 1 1984|
ASJC Scopus subject areas
- Cancer Research