Heightened Interferon-γ Production by Mononuclear Cells from Bladder Cancer Patients

Timothy L. Ratliff*, William J. Catalona, David R. Kelley, Amos Shapiro

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

lnterferon-$gM (IFN-$gM) production by peripheral blood leukocytes from bladder cancer patients was compared with that of patients with prostate cancer and benign prostatic hyperplasia, non-tumor-bearing patients with bacterial infections, and normal controls. Leukocyte preparations including mononuclear cells isolated on a Ficoll-Hypaque density gradient (Fraction 2) and glass-nonadherent mononuclear cells (Fraction 3) were stimulated with Protein A from Staphylococcus aureus, and IFN-$gM production was monitored 24 hr late r. The class of interferon produced was identified by antibody neutralization experiments which clearly showed S. aureus Protein A-induced interferon to be IFN-$gM. There was significantly heightened IFN-$gM production by Fraction 3 cells from bladder cancer patients and patients with bacterial infections. Heightened IFN-$gM production by bladder cancer patients was not observed in the Fraction 2 cells. No correlation was observed between IFN-$gM production and patients with invasive or noninvasive bladder cancer, but IFN-$gM production was lower in patients having Stage C or D tumors than in those having Stage A or B tumors. These results in conjunction with previous reports demonstrating heightened IFN-$gM production during periods of antigenic stimulation suggest that bladder tumors may induce a cell-mediated immune response in the host as evidenced by the elevation in IFN-$gM production. Moreover, the results suggest that macrophages may be important regulators of IFN-$gM production in bladder cancer patients.

Original languageEnglish (US)
Pages (from-to)3140-3143
Number of pages4
JournalCancer Research
Volume44
Issue number7
StatePublished - Jul 1 1984

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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