Hematogones are markedly reduced in pediatric acquired aplastic anemia: Multiparametric flow cytometric analysis

Deborah W. Sevilla, Foxwell N. Emmons, Adriana I. Colovai, Nike T. Beaubier, Courtney Baker, Monica Bhatia, Govind Bhagat, Bachir Alobeid*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Acquired aplastic anemia (AA) and myelodysplastic syndromes (MDS) are bone marrow (BM) failure syndromes with overlapping clinical features, and at least a subset appears to share common pathophysiologic mechanisms. Recent studies of MDS have shown down-regulation of genes involved in B-cell development and decreased B-cell precursors (hematogones). We explored the possibility that AA, similar to MDS, might also be associated with defects in development of lymphoid cells, especially B-cells, by using flow cytometry to assess the presence of hematogones and mature lymphocytes in BM samples from 25 children with AA and 41 age-matched controls. We observed that the percentage of total and early (stage I) hematogones were significantly decreased in AA compared to controls, and they returned to normal numbers after hematopoietic stem-cell transplant. This demonstrates early B-cell lineage involvement in AA, similar to recent findings in MDS. Our findings suggest dysfunction of an early multilineage progenitor in the pathogenesis of AA.

Original languageEnglish (US)
Pages (from-to)1951-1957
Number of pages7
JournalLeukemia and Lymphoma
Volume50
Issue number12
DOIs
StatePublished - Dec 1 2009

Keywords

  • Aplastic anemia
  • B-cells, NK-cells
  • CD8+ T-cells
  • Flow cytometry
  • Hematogones
  • Myelodysplastic syndrome
  • Precursor B-cells

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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