Hematopoietic cell culture therapies (Part I): Cell culture considerations

Todd A. McAdams*, William M. Miller, E. Terry Papoutsakis

*Corresponding author for this work

Research output: Contribution to journalReview article

41 Citations (Scopus)

Abstract

Hematopoietic cell culture, or ex vivo expansion of hematopoietic cells, is a rapidly growing area of tissue engineering with many potential applications in bone-marrow transplantation, gene therapy and the production of blood products. Hematopoietic cultures are considerably more complex than established animal cell culture technologies owing to the presence of many cell types at various differentiation stages, and stringent medium and growth factor requirements. Culture parameters, such as pH, oxygen tension, seeding density and feeding schedules, significantly affect the proliferation and differentiation of hematopoietic cells. A number of bioreactor systems are currently under development.

Original languageEnglish (US)
Pages (from-to)341-349
Number of pages9
JournalTrends in biotechnology
Volume14
Issue number9
DOIs
StatePublished - Sep 1996

Fingerprint

Cell- and Tissue-Based Therapy
Cell culture
GTP Pyrophosphokinase
Animal cell culture
Cell Culture Techniques
Gene therapy
Bioelectric potentials
Bioreactors
Tissue Engineering
Bone Marrow Transplantation
Tissue engineering
Genetic Therapy
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Appointments and Schedules
Bone
Blood
Cells
Oxygen
Technology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

McAdams, Todd A. ; Miller, William M. ; Papoutsakis, E. Terry. / Hematopoietic cell culture therapies (Part I) : Cell culture considerations. In: Trends in biotechnology. 1996 ; Vol. 14, No. 9. pp. 341-349.
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Hematopoietic cell culture therapies (Part I) : Cell culture considerations. / McAdams, Todd A.; Miller, William M.; Papoutsakis, E. Terry.

In: Trends in biotechnology, Vol. 14, No. 9, 09.1996, p. 341-349.

Research output: Contribution to journalReview article

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