Hematopoietic cell culture therapies (Part I): Cell culture considerations

Todd A. McAdams; William M. Miller; E. Terry Papoutsakis

doi:10.1016/0167-7799(96)10047-0

Hematopoietic cell culture therapies (Part I): Cell culture considerations

Todd A. McAdams^*, William M. Miller, E. Terry Papoutsakis

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

43 Scopus citations

Abstract

Hematopoietic cell culture, or ex vivo expansion of hematopoietic cells, is a rapidly growing area of tissue engineering with many potential applications in bone-marrow transplantation, gene therapy and the production of blood products. Hematopoietic cultures are considerably more complex than established animal cell culture technologies owing to the presence of many cell types at various differentiation stages, and stringent medium and growth factor requirements. Culture parameters, such as pH, oxygen tension, seeding density and feeding schedules, significantly affect the proliferation and differentiation of hematopoietic cells. A number of bioreactor systems are currently under development.

Original language	English (US)
Pages (from-to)	341-349
Number of pages	9
Journal	Trends in biotechnology
Volume	14
Issue number	9
DOIs	https://doi.org/10.1016/0167-7799(96)10047-0
State	Published - Sep 1996
Externally published	Yes

Funding

Acknowledgements This work is supportedb y NationalI nstituteso f Health (USA) Grant R01 HL48276. T. A. M. was 39 partiallys upportedb y a PredoctoraBl iotechnology 40 Training grant( NIH GM08449). 41

ASJC Scopus subject areas

Bioengineering
Biotechnology

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title = "Hematopoietic cell culture therapies (Part I): Cell culture considerations",

abstract = "Hematopoietic cell culture, or ex vivo expansion of hematopoietic cells, is a rapidly growing area of tissue engineering with many potential applications in bone-marrow transplantation, gene therapy and the production of blood products. Hematopoietic cultures are considerably more complex than established animal cell culture technologies owing to the presence of many cell types at various differentiation stages, and stringent medium and growth factor requirements. Culture parameters, such as pH, oxygen tension, seeding density and feeding schedules, significantly affect the proliferation and differentiation of hematopoietic cells. A number of bioreactor systems are currently under development.",

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note = "Funding Information: Acknowledgements This work is supportedb y NationalI nstituteso f Health (USA) Grant R01 HL48276. T. A. M. was 39 partiallys upportedb y a PredoctoraBl iotechnology 40 Training grant( NIH GM08449). 41",

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AU - Miller, William M.

AU - Papoutsakis, E. Terry

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AB - Hematopoietic cell culture, or ex vivo expansion of hematopoietic cells, is a rapidly growing area of tissue engineering with many potential applications in bone-marrow transplantation, gene therapy and the production of blood products. Hematopoietic cultures are considerably more complex than established animal cell culture technologies owing to the presence of many cell types at various differentiation stages, and stringent medium and growth factor requirements. Culture parameters, such as pH, oxygen tension, seeding density and feeding schedules, significantly affect the proliferation and differentiation of hematopoietic cells. A number of bioreactor systems are currently under development.

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Hematopoietic cell culture therapies (Part I): Cell culture considerations

Abstract

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