Heme oxygenase-1 expression protects melanocytes from stress-induced cell death: Implications for vitiligo

Yasser E. Elassiuty, Jared Klarquist, Jodi Speiser, Randa M. Yousef, Abdelaziz A. El Refaee, Nahla S. Hunter, Olfat G. Shaker, Mohan Gundeti, Ludmila Nieuweboer-Krobotova, I. Caroline Le Poole*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

To study protection of melanocytes from stress-induced cell death by heme oxygenases during depigmentation and repigmentation in vitiligo, expression of isoforms 1 and 2 was studied in cultured control and patient melanocytes and normal skin explants exposed to UV or bleaching agent 4-TBP. Similarly, expression of heme oxygenases was followed in skin from vitiligo patients before and after PUVA treatment. Single and double immunostainings were used in combination with light and confocal microscopic analysis and Western blotting. Melanocyte expression of heme oxygenase 1 is upregulated, whereas heme oxygenase 2 is reduced in response to UV and 4-TBP. Upregulation of inducible heme oxygenase 1 was also observed in UV-treated explant cultures, in skin of successfully PUVA-treated patients and in melanocytes cultured from vitiligo non-lesional skin. Heme oxygenase encoding genes were subsequently cloned to study consequences of either gene product on cell viability, demonstrating that HO-1 but not HO-2 overexpression offers protection from stress-induced cell death in MTT assays. HO-1 expression by melanocytes may contribute to beneficial effects of UV treatment for vitiligo patients.

Original languageEnglish (US)
Pages (from-to)496-501
Number of pages6
JournalExperimental Dermatology
Volume20
Issue number6
DOIs
StatePublished - Jun 2011

Keywords

  • Heme oxygenase
  • Melanocyte
  • Stress
  • Ultraviolet

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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