Heparin-mediated antibiotic delivery from an electrochemically-aligned collagen sheet

Olivia T. Cheng, Andrew P. Stein, Eric Babajanian, Kathryn R. Hoppe, Shawn Li, Hyungjin Jung, Anish Abrol, Anna Akkus, Mousa Younesi, Ghaith Altawallbeh, Mahmoud A. Ghannoum, Tracey Bonfield, Ozan Akkus, Chad A. Zender*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Implantable medical devices and hardware are prolific in medicine, but hardware associated infections remain a major issue. Objective: To develop and evaluate a novel, biologic antimicrobial coating for medical implants. Methods: Electrochemically compacted collagen sheets with and without crosslinked heparin were synthesized per a protocol developed by our group. Sheets were incubated in antibiotic solution (gentamicin or moxifloxacin) overnight, and in vitro activity was assessed with five-day diffusion assays against Pseudomonas aeruginosa. Antibiotic release over time from gentamicin-infused sheets was determined using in vitro elution and high performance liquid chromatography (HPLC). Results: Collagen-heparin-antibiotic sheets demonstrated larger growth inhibition zones against P. aeruginosa compared to collagen-antibiotic alone sheets. This activity persisted for five days and was not impacted by rinsing sheets prior to evaluation. Rinsed collagen-antibiotic sheets did not produce any inhibition zones. Elution of gentamicin from collagen-heparin-gentamicin sheets was gradual and remained above the minimal inhibitory concentration for gentamicin-sensitive organisms for 29 days. Conversely, collagen-gentamicin sheets eluted their antibiotic load within 24 hours. Overall, heparin-associated sheets demonstrated larger inhibition zones against P. aeruginosa and prolonged elution profile via HPLC. Conclusion: We developed a novel, local antibiotic delivery system that could be used to coat medical implants/hardware in the future and reduce post-operative infections.

Original languageEnglish (US)
Pages (from-to)159-170
Number of pages12
JournalBio-Medical Materials and Engineering
Volume32
Issue number3
DOIs
StatePublished - 2021
Externally publishedYes

Keywords

  • collagen sheet with heparin
  • electrochemically compacted collagen sheet
  • implantable medical devices and hardware
  • Local antibiotic delivery
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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