Abstract
Background: Implantable medical devices and hardware are prolific in medicine, but hardware associated infections remain a major issue. Objective: To develop and evaluate a novel, biologic antimicrobial coating for medical implants. Methods: Electrochemically compacted collagen sheets with and without crosslinked heparin were synthesized per a protocol developed by our group. Sheets were incubated in antibiotic solution (gentamicin or moxifloxacin) overnight, and in vitro activity was assessed with five-day diffusion assays against Pseudomonas aeruginosa. Antibiotic release over time from gentamicin-infused sheets was determined using in vitro elution and high performance liquid chromatography (HPLC). Results: Collagen-heparin-antibiotic sheets demonstrated larger growth inhibition zones against P. aeruginosa compared to collagen-antibiotic alone sheets. This activity persisted for five days and was not impacted by rinsing sheets prior to evaluation. Rinsed collagen-antibiotic sheets did not produce any inhibition zones. Elution of gentamicin from collagen-heparin-gentamicin sheets was gradual and remained above the minimal inhibitory concentration for gentamicin-sensitive organisms for 29 days. Conversely, collagen-gentamicin sheets eluted their antibiotic load within 24 hours. Overall, heparin-associated sheets demonstrated larger inhibition zones against P. aeruginosa and prolonged elution profile via HPLC. Conclusion: We developed a novel, local antibiotic delivery system that could be used to coat medical implants/hardware in the future and reduce post-operative infections.
Original language | English (US) |
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Pages (from-to) | 159-170 |
Number of pages | 12 |
Journal | Bio-Medical Materials and Engineering |
Volume | 32 |
Issue number | 3 |
DOIs | |
State | Published - 2021 |
Keywords
- Local antibiotic delivery
- Pseudomonas aeruginosa
- collagen sheet with heparin
- electrochemically compacted collagen sheet
- implantable medical devices and hardware
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering