TY - JOUR
T1 - Heparin-mediated antibiotic delivery from an electrochemically-aligned collagen sheet
AU - Cheng, Olivia T.
AU - Stein, Andrew P.
AU - Babajanian, Eric
AU - Hoppe, Kathryn R.
AU - Li, Shawn
AU - Jung, Hyungjin
AU - Abrol, Anish
AU - Akkus, Anna
AU - Younesi, Mousa
AU - Altawallbeh, Ghaith
AU - Ghannoum, Mahmoud A.
AU - Bonfield, Tracey
AU - Akkus, Ozan
AU - Zender, Chad A.
N1 - Publisher Copyright:
© 2021 - IOS Press. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Background: Implantable medical devices and hardware are prolific in medicine, but hardware associated infections remain a major issue. Objective: To develop and evaluate a novel, biologic antimicrobial coating for medical implants. Methods: Electrochemically compacted collagen sheets with and without crosslinked heparin were synthesized per a protocol developed by our group. Sheets were incubated in antibiotic solution (gentamicin or moxifloxacin) overnight, and in vitro activity was assessed with five-day diffusion assays against Pseudomonas aeruginosa. Antibiotic release over time from gentamicin-infused sheets was determined using in vitro elution and high performance liquid chromatography (HPLC). Results: Collagen-heparin-antibiotic sheets demonstrated larger growth inhibition zones against P. aeruginosa compared to collagen-antibiotic alone sheets. This activity persisted for five days and was not impacted by rinsing sheets prior to evaluation. Rinsed collagen-antibiotic sheets did not produce any inhibition zones. Elution of gentamicin from collagen-heparin-gentamicin sheets was gradual and remained above the minimal inhibitory concentration for gentamicin-sensitive organisms for 29 days. Conversely, collagen-gentamicin sheets eluted their antibiotic load within 24 hours. Overall, heparin-associated sheets demonstrated larger inhibition zones against P. aeruginosa and prolonged elution profile via HPLC. Conclusion: We developed a novel, local antibiotic delivery system that could be used to coat medical implants/hardware in the future and reduce post-operative infections.
AB - Background: Implantable medical devices and hardware are prolific in medicine, but hardware associated infections remain a major issue. Objective: To develop and evaluate a novel, biologic antimicrobial coating for medical implants. Methods: Electrochemically compacted collagen sheets with and without crosslinked heparin were synthesized per a protocol developed by our group. Sheets were incubated in antibiotic solution (gentamicin or moxifloxacin) overnight, and in vitro activity was assessed with five-day diffusion assays against Pseudomonas aeruginosa. Antibiotic release over time from gentamicin-infused sheets was determined using in vitro elution and high performance liquid chromatography (HPLC). Results: Collagen-heparin-antibiotic sheets demonstrated larger growth inhibition zones against P. aeruginosa compared to collagen-antibiotic alone sheets. This activity persisted for five days and was not impacted by rinsing sheets prior to evaluation. Rinsed collagen-antibiotic sheets did not produce any inhibition zones. Elution of gentamicin from collagen-heparin-gentamicin sheets was gradual and remained above the minimal inhibitory concentration for gentamicin-sensitive organisms for 29 days. Conversely, collagen-gentamicin sheets eluted their antibiotic load within 24 hours. Overall, heparin-associated sheets demonstrated larger inhibition zones against P. aeruginosa and prolonged elution profile via HPLC. Conclusion: We developed a novel, local antibiotic delivery system that could be used to coat medical implants/hardware in the future and reduce post-operative infections.
KW - collagen sheet with heparin
KW - electrochemically compacted collagen sheet
KW - implantable medical devices and hardware
KW - Local antibiotic delivery
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=85106869804&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85106869804&partnerID=8YFLogxK
U2 - 10.3233/BME-201133
DO - 10.3233/BME-201133
M3 - Article
C2 - 33780355
AN - SCOPUS:85106869804
SN - 0959-2989
VL - 32
SP - 159
EP - 170
JO - Bio-Medical Materials and Engineering
JF - Bio-Medical Materials and Engineering
IS - 3
ER -