Hepatitis C testing: Comparison of Ortho's EIA and RIBA II tests in 1,182 patients undergoing primary liver transplantation

M. Rochlani, J. H. Lewis*, G. E. Ramsey, F. A. Bontempo, G. Shah, R. A. Bowman, D. H. Van Thiel, T. E. Starzl

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Plasma samples from 1,182 patients undergoing primary liver transplantation were tested for anti-hepatitis C (HCV) virus by two methods: Ortho HCV ELISA Test System (EIA) and Chiron RIBA HCV Test System (RIBA II). The EIA results, 0 or +, were recorded first, followed by RIBA results, N = negative, P = positive, or I = indeterminate. Concordant results-ON, + P, + I-were found in 1,076 (91%), and discordant results were found in 106 (9%). The EIA optical density did not relate to concordant or discordant results. Band patterns were described by stating the band position (1, 2, 3, or 4) and inserting a dash (-) if no band was visualized. Most + P samples fell into two patterns: 47% showed all four bands, pattern 1234, and 15% showed the two-band pattern, 34. When the EIA was negative, OP, the opposite was seen: 8% showed the 1234 pattern and 81% showed the 34 pattern. There were 226 samples that formed bands (+ P, 149; OP, 31; + I, 15; OI, 31). The frequency of bands was as follows: 4, 32%; 3, 31%; 2, 19%; and 1, 18%. Band 2 and the EIA test detected antibodies to the same c100-3 fragment and showed 74% concordance. No explanation is apparent for the lower concordance rate here than that between the EIA test and bands 3 = 96% or 4 = 88%. The EIA and RIBA II tests, together with positive liver function tests and abnormal tissue pathologic findings, provide a basis for the diagnosis of HCV.

Original languageEnglish (US)
Pages (from-to)8-12
Number of pages5
JournalAmerican journal of clinical pathology
Volume98
Issue number1
DOIs
StatePublished - 1992

Keywords

  • Anti-HCV
  • Hepatitis C
  • Non-A,non-B hepatitis
  • RIBA II band patterns

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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