Hepatocellular ultrastructure after ischemia/reperfusion injury in human orthotopic liver transplantation

Satish N. Nadig; Basker Periyasamy; Stephen F. Shafizadeh; Carmen Polito; Ryan N. Fiorini; David Rodwell; Zachary Evans; Gang Cheng; Dana Dunkelberger; Michael Schmidt; Sally E. Self; Kenneth D. Chavin

doi:10.1016/j.gassur.2004.04.002

Hepatocellular ultrastructure after ischemia/reperfusion injury in human orthotopic liver transplantation

Satish N. Nadig, Basker Periyasamy, Stephen F. Shafizadeh, Carmen Polito, Ryan N. Fiorini, David Rodwell, Zachary Evans, Gang Cheng, Dana Dunkelberger, Michael Schmidt, Sally E. Self, Kenneth D. Chavin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The number of patients requiring organ transplants still outpaces the number of available transplantable organs. During the process of orthotopic liver transplantation (OLTx), donor organs undergo significant stress resulting from ischemia and reperfusion. Healthy organs respond to this stressful environment with compensatory mechanisms that ideally allow for complete recovery. However, "marginal" organs do not compensate as well. Hepatic steatosis typically renders an organ nontransplantable; a liver with 30% or more fat has a 25% chance of primary nonfunction (PNF) or graft failure after a technically sound operation. In this study, we report on the significant markers of cellular ultrastructural change in steatotic livers. These include glycogen content, mitochondrial swelling, and hepatocellular blebbing. The data disclosed here argue that further investigation of these factors in marginal organs subjected to I/R may better facilitate our understanding of PNF.

Original language	English (US)
Pages (from-to)	695-700
Number of pages	6
Journal	Journal of Gastrointestinal Surgery
Volume	8
Issue number	6
DOIs	https://doi.org/10.1016/j.gassur.2004.04.002
State	Published - Sep 10 2004
Externally published	Yes

Keywords

Mitochondrial swelling
primary nonfunction
steatosis
transmission electron microscopy (TEM)
ultrastructural changes

ASJC Scopus subject areas

Surgery
Gastroenterology

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10.1016/j.gassur.2004.04.002

Cite this

Nadig, S. N., Periyasamy, B., Shafizadeh, S. F., Polito, C., Fiorini, R. N., Rodwell, D., Evans, Z., Cheng, G., Dunkelberger, D., Schmidt, M., Self, S. E., & Chavin, K. D. (2004). Hepatocellular ultrastructure after ischemia/reperfusion injury in human orthotopic liver transplantation. Journal of Gastrointestinal Surgery, 8(6), 695-700. https://doi.org/10.1016/j.gassur.2004.04.002

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abstract = "The number of patients requiring organ transplants still outpaces the number of available transplantable organs. During the process of orthotopic liver transplantation (OLTx), donor organs undergo significant stress resulting from ischemia and reperfusion. Healthy organs respond to this stressful environment with compensatory mechanisms that ideally allow for complete recovery. However, {"}marginal{"} organs do not compensate as well. Hepatic steatosis typically renders an organ nontransplantable; a liver with 30% or more fat has a 25% chance of primary nonfunction (PNF) or graft failure after a technically sound operation. In this study, we report on the significant markers of cellular ultrastructural change in steatotic livers. These include glycogen content, mitochondrial swelling, and hepatocellular blebbing. The data disclosed here argue that further investigation of these factors in marginal organs subjected to I/R may better facilitate our understanding of PNF.",

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Nadig, SN, Periyasamy, B, Shafizadeh, SF, Polito, C, Fiorini, RN, Rodwell, D, Evans, Z, Cheng, G, Dunkelberger, D, Schmidt, M, Self, SE & Chavin, KD 2004, 'Hepatocellular ultrastructure after ischemia/reperfusion injury in human orthotopic liver transplantation', Journal of Gastrointestinal Surgery, vol. 8, no. 6, pp. 695-700. https://doi.org/10.1016/j.gassur.2004.04.002

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AU - Periyasamy, Basker

AU - Shafizadeh, Stephen F.

AU - Polito, Carmen

AU - Fiorini, Ryan N.

AU - Rodwell, David

AU - Evans, Zachary

AU - Cheng, Gang

AU - Dunkelberger, Dana

AU - Schmidt, Michael

AU - Self, Sally E.

AU - Chavin, Kenneth D.

PY - 2004/9/10

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N2 - The number of patients requiring organ transplants still outpaces the number of available transplantable organs. During the process of orthotopic liver transplantation (OLTx), donor organs undergo significant stress resulting from ischemia and reperfusion. Healthy organs respond to this stressful environment with compensatory mechanisms that ideally allow for complete recovery. However, "marginal" organs do not compensate as well. Hepatic steatosis typically renders an organ nontransplantable; a liver with 30% or more fat has a 25% chance of primary nonfunction (PNF) or graft failure after a technically sound operation. In this study, we report on the significant markers of cellular ultrastructural change in steatotic livers. These include glycogen content, mitochondrial swelling, and hepatocellular blebbing. The data disclosed here argue that further investigation of these factors in marginal organs subjected to I/R may better facilitate our understanding of PNF.

AB - The number of patients requiring organ transplants still outpaces the number of available transplantable organs. During the process of orthotopic liver transplantation (OLTx), donor organs undergo significant stress resulting from ischemia and reperfusion. Healthy organs respond to this stressful environment with compensatory mechanisms that ideally allow for complete recovery. However, "marginal" organs do not compensate as well. Hepatic steatosis typically renders an organ nontransplantable; a liver with 30% or more fat has a 25% chance of primary nonfunction (PNF) or graft failure after a technically sound operation. In this study, we report on the significant markers of cellular ultrastructural change in steatotic livers. These include glycogen content, mitochondrial swelling, and hepatocellular blebbing. The data disclosed here argue that further investigation of these factors in marginal organs subjected to I/R may better facilitate our understanding of PNF.

KW - Mitochondrial swelling

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KW - steatosis

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Hepatocellular ultrastructure after ischemia/reperfusion injury in human orthotopic liver transplantation

Abstract

Keywords

ASJC Scopus subject areas

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