Hepatocyte growth factor/scatter factor-met signaling induces proliferation, migration, and morphogenesis of pancreatic oval cells

Michael Jeffers, M. Sambasiva Rao, Shen Rulong, Janardan K. Reddy, Vadrevu Subbarao, Eric Hudson, George F. Vande Woude, James H. Resau*

*Corresponding author for this work

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic effector for cells expressing the Met tyrosine kinase receptor. In this investigation, we show that pancreatic oval cells express Met and exhibit a proliferative response to HGF/SF. Additionally, we found that oval cells treated transiently with this factor become 'scattered,' whereas those exposed to HGF/SF for extended periods of time form branching tubular structures. These structures possess true lumens, which are lined by cells with ductal features, including apical microvilli, well-developed intercellular junctions, interdigitation of plasma membranes, and abundant cytoplasmic organelles, interestingly, these ductal structures are formed by HGF/SF-treated cells cultured on plastic dishes in the absence of exogenous extracellular matrix components. Consistent with their ability to form ductal structures in vitro, we found that pancreatic oval cells form ductal adenocarcinomas in nude mice. This study supports the involvement of HGF/SF- Met signaling in the growth, migration, and morphogenesis of pancreatic oval cells and may have important implications for the expansion and morphogenic differentiation of these cells during developmental, regenerative, and neoplastic growth.

Original languageEnglish (US)
Pages (from-to)1805-1813
Number of pages9
JournalCell Growth and Differentiation
Volume7
Issue number12
StatePublished - Dec 21 1996

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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