Her-2/neu expression in breast cancer - A comparison of different diagnostic methods

Peter Benöhr*, V. Henkel, R. Speer, U. Vogel, K. Sotlar, B. Aydeniz, A. Reiser, H. Neubauer, K. Tabiti, D. Wallwiener, S. E. Clare, R. Kurek

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Background: Determination of Her-2/neu overexpression in breast cancer has previously been shown to be of prognostic significance. In this study, Her-2/neu expression in breast cancer was characterised by real-time PCR (RLT-PCR) based LightCycler-HER-2/neu DNA Quantification with immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH). Material and Methods: Fifteen specimens of invasive breast cancer - whole tissue sections as well as microdissected tumour cells - were subjected to RLT-PCR. Additionally, IHC and FISH were performed. Results: Her-2/neu overexpression was detected by FISH and by real-time PCR in the same tumours. In contrast, IHC revealed discordant results. Conclusion: Determination of Her-2/neu amplification by real-time PCR is a sensitive and specific method with some advantages over FISH. This method is simple and reliable and has the potential of categorizing those tumours with borderline Her-2/neu overexpression as determined by IHC.

Original languageEnglish (US)
Pages (from-to)1895-1900
Number of pages6
JournalAnticancer Research
Volume25
Issue number3 B
StatePublished - May 1 2005

Keywords

  • Breast cancer
  • Her-2/neu
  • Real-time PCR

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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