Heterogeneity in plasminogen activator (PA) levels in human prostate cancer cell lines: Increased PA activity correlates with biologically aggressive behavior

Harold N. Keer, Franklin D. Gaylis, James M. Kozlowski*, Hau C. Kwaan, Kenneth D. Bauer, Akhouri A. Sinha, Michael J. Wilson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Plasminogen activators (PA), particularly the lower Mr urokinase (u‐PA) type, have been associated with tumor cell invasion and metastasis. We have examined the expression of PA by two human prostate cancer cell lines (PC‐3 and DU‐145) using functional and immunologic techniques. The culture media and cell extracts of the more aggressive PC‐3 cell line contained more than two‐fold greater PA activity than the relatively indolent DU‐145 cell line. Zymographic studies identified the PA expressed as u‐PA. PC‐3 cells expressed an additional lower molecular weight form of u‐PA not noted in DU‐145 cells. Heterogeneity in u‐PA expression was shown by the fibrin lysis assay, immunohistochemistry, and dual parameter flow cytometry indicating the presence of phenotypically divergent cell populations. Increased u‐PA expression may identify those tumor cells that possess aggressive biological potential.

Original languageEnglish (US)
Pages (from-to)201-214
Number of pages14
JournalThe Prostate
Volume18
Issue number3
DOIs
StatePublished - 1991

Keywords

  • invasion
  • metastasis
  • urokinase

ASJC Scopus subject areas

  • Oncology
  • Urology

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