Hfoapp: A matlab graphical user interface for high-frequency oscillation marking

Guangyu Zhou*, Torben Noto, Arjun Sharma, Qiaohan Yang, Karina A. González Otárula, Matthew Tate, Jessica W. Templer, Gregory Lane, Christina Zelano

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Epilepsy affects 3.4 million people in the United States, and, despite the availability of numerous antiepileptic drugs, 36% of patients have uncontrollable seizures, which severely impact quality of life. High-frequency oscillations (HFOs) are a potential biomarker of epileptogenic tissue that could be useful in surgical planning. As a result, research into the efficacy of HFOs as a clinical tool has increased over the last 2 decades. However, detection and identification of these transient rhythms in intracranial electroencephalographic recordings re-main time-consuming and challenging. Although automated detection algorithms have been developed, their results are widely inconsistent, reducing reliability. Thus, manual marking of HFOs remains the gold standard, and manual review of automated results is required. However, manual marking and review are time consuming and can still produce variable results because of their subjective nature and the limitations in functionality of existing open-source software. Our goal was to develop a new software with broad application that improves on existing open-source HFO detection applications in usability, speed, and accuracy. Here, we present HFOApp: a free, open-source, easy-to-use MATLAB-based graphical user interface for HFO marking. This toolbox offers a high degree of intuitive and ergonomic usability and integrates interactive automation-assist options with manual marking, significantly reducing the time needed for review and manual marking of recordings, while increasing interrater reliability. The toolbox also features simultaneous multichannel detection and marking. HFOApp was designed as an easy-to-use toolbox for clinicians and researchers to quickly and accu-rately mark, quantify, and characterize HFOs within electrophysiological datasets.

Original languageEnglish (US)
Article numberENEURO.0509-20.2021
Issue number5
StatePublished - 2021


  • Graphical user interface
  • High-frequency oscillations

ASJC Scopus subject areas

  • Neuroscience(all)


Dive into the research topics of 'Hfoapp: A matlab graphical user interface for high-frequency oscillation marking'. Together they form a unique fingerprint.

Cite this