TY - JOUR
T1 - High molecular weight collagen
T2 - A long-lived intermediate in the biogenesis of collagen fibrils
AU - Veis, Arthur
AU - Anesey, Joan R.
AU - Garvin, James E.
AU - Dimuzio, Michael T.
PY - 1972/9/26
Y1 - 1972/9/26
N2 - CM-cellulose and Agarose chromatography of denatured rat skin collagen show it to contain α1-chains in two distinct molecular weight classes: (α1)l, 1 × 105 daltons; (α1)h, 1.1 × 105 daltons. Pulse labeling in vivo with 3H-proline (G) for short periods, 90 and 120 minutes, shows the (α1)h-chains of acid soluble collage to have nearly double the specific activity of the (α1)l-chains, indicating a precursor-product relationship. However, unlabeled (α1)h has been isolated from the skins by successive extractions, indicating that conversion of (α1)h to (α1)l is neither immediate nor, possibly, complete. Since (α1)h is smaller than the pro-α1 chains of nascent procollagen, it is clear that procollagen is not cleaved in vivo in a single step to collagen. Heightened aggregation properties of the intermediate collagens containing (α1)h suggest that they may have a particular role in fibrillogenesis.
AB - CM-cellulose and Agarose chromatography of denatured rat skin collagen show it to contain α1-chains in two distinct molecular weight classes: (α1)l, 1 × 105 daltons; (α1)h, 1.1 × 105 daltons. Pulse labeling in vivo with 3H-proline (G) for short periods, 90 and 120 minutes, shows the (α1)h-chains of acid soluble collage to have nearly double the specific activity of the (α1)l-chains, indicating a precursor-product relationship. However, unlabeled (α1)h has been isolated from the skins by successive extractions, indicating that conversion of (α1)h to (α1)l is neither immediate nor, possibly, complete. Since (α1)h is smaller than the pro-α1 chains of nascent procollagen, it is clear that procollagen is not cleaved in vivo in a single step to collagen. Heightened aggregation properties of the intermediate collagens containing (α1)h suggest that they may have a particular role in fibrillogenesis.
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U2 - 10.1016/0006-291X(72)90869-8
DO - 10.1016/0006-291X(72)90869-8
M3 - Article
C2 - 5077827
AN - SCOPUS:0015525358
SN - 0006-291X
VL - 48
SP - 1404
EP - 1411
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 6
ER -