High molecular weight protein MAP 2 promoting microtubuleassembly in vitro is associated with microtubules in cells

S. A. Kuznetsov*, V. I. Rodionov, A. D. Bershadsky, V. I. Gelfand, V. A. Rosenblat

*Corresponding author for this work

Research output: Contribution to journalArticle

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Abstract

Brain high molecular weight (HMW) protein promoting microtubule assembly in vitro and identical to MAP 2 (one of the proteins which copurify with tubulin through microtubule assembly-disassembly cycles), is shown to be associated with microtubules in interphase and mitotic cells. This HMW protein was purified earlier (Kuznetsov et al., 1978), directly from bovine brain without previous obtaining total microtubule protein. Now we have obtained a monospecific antibody against it. Identity of the HMW protein with MAP 2 is inferred from SDS-electrophoresis and immunological tests; its intra-cellular localization is determined by indirect immunofluorescent staining of cultured bovine tracheal epithelium.The anti-MAP 2 antibody stains the same structures in the cells as the tubulin antibody: it stains the fibrillar network in interphase cells, mitotic spindle, and the stem body. No fibrillar structures in the cells treated with colchicine or vinblastine were stained with the antibody against MAP 2. Anti-MAP 2 also stains tubulin-containing paracrystals which have been formed in the vinblastine-treated cells. Therefore HMW protein MAP 2 which promotes tubulin polymerization in vitro is associated with microtubules in vivo.

Original languageEnglish (US)
Pages (from-to)1017-1024
Number of pages8
JournalCell Biology International Reports
Volume4
Issue number11
DOIs
StatePublished - Jan 1 1980

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Microtubules
Tubulin
Molecular Weight
Microtubule Proteins
Coloring Agents
Vinblastine
Antibodies
Interphase
Proteins
Immunologic Tests
Spindle Apparatus
Colchicine
Brain
Polymerization
Electrophoresis
Epithelium
In Vitro Techniques
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kuznetsov, S. A. ; Rodionov, V. I. ; Bershadsky, A. D. ; Gelfand, V. I. ; Rosenblat, V. A. / High molecular weight protein MAP 2 promoting microtubuleassembly in vitro is associated with microtubules in cells. In: Cell Biology International Reports. 1980 ; Vol. 4, No. 11. pp. 1017-1024.
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High molecular weight protein MAP 2 promoting microtubuleassembly in vitro is associated with microtubules in cells. / Kuznetsov, S. A.; Rodionov, V. I.; Bershadsky, A. D.; Gelfand, V. I.; Rosenblat, V. A.

In: Cell Biology International Reports, Vol. 4, No. 11, 01.01.1980, p. 1017-1024.

Research output: Contribution to journalArticle

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