High-order fluorescence and exciton interaction in photosynthetic bacteria

Mayfair Chu Kung; Don Devault

doi:10.1016/0005-2728(78)90028-2

High-order fluorescence and exciton interaction in photosynthetic bacteria

Mayfair Chu Kung, Don Devault^*

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We have observed fluorescence at visible wavelengths from chromatophores of photosynthetic bacteria excited with infrared radiation which we attribute to bacteriochlorophyll of the antenna system. The fluorescence is prompt (no delay greater than 5 ns). Its spectrum shows peaks at 445, 530 (broad) and 600 nm when excited with either 694 or 868 nm. Quantum yield is of the order of 10^-9. The dependence on intensity indicates generation by mainly third-order processes which could involve triplet states in combination with excited singlets. Second-order single-singlet fusion could also contribute. The high-order fluorescence can also be explained as arising from absorption of a second photon by singlet excited states.

Original language	English (US)
Pages (from-to)	217-231
Number of pages	15
Journal	BBA - Bioenergetics
Volume	501
Issue number	2
DOIs	https://doi.org/10.1016/0005-2728(78)90028-2
State	Published - Feb 9 1978

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(78)90028-2

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@article{cd0f2ea9946b4c3e8fc6ba328f6f062b,

title = "High-order fluorescence and exciton interaction in photosynthetic bacteria",

abstract = "We have observed fluorescence at visible wavelengths from chromatophores of photosynthetic bacteria excited with infrared radiation which we attribute to bacteriochlorophyll of the antenna system. The fluorescence is prompt (no delay greater than 5 ns). Its spectrum shows peaks at 445, 530 (broad) and 600 nm when excited with either 694 or 868 nm. Quantum yield is of the order of 10-9. The dependence on intensity indicates generation by mainly third-order processes which could involve triplet states in combination with excited singlets. Second-order single-singlet fusion could also contribute. The high-order fluorescence can also be explained as arising from absorption of a second photon by singlet excited states.",

author = "Kung, {Mayfair Chu} and Don Devault",

note = "Funding Information: The authors wish to thank Dr. P.L. Dutton and his coworkers for the samples of R. sphaeroides and some of the chromatophores. A number of people, among whom may be mentioned M. Gouterman, D. Mauzerall, W.W. Parson, P.A. Loach, Govindjee, K.J. Kaufmann, C.E. Swenberg, and R.M. Hochstrasser have contributed helpful discussions. Mr. D. Henderson helped with the apparatus. We also thank Dr. William Parson for sending us several of his manuscripts before publication. Financial support came from the National Science Foundation (grants PCM76-15724 and PCM76-23744).",

year = "1978",

month = feb,

day = "9",

doi = "10.1016/0005-2728(78)90028-2",

language = "English (US)",

volume = "501",

pages = "217--231",

journal = "BBA - Bioenergetics",

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number = "2",

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TY - JOUR

T1 - High-order fluorescence and exciton interaction in photosynthetic bacteria

AU - Kung, Mayfair Chu

AU - Devault, Don

N1 - Funding Information: The authors wish to thank Dr. P.L. Dutton and his coworkers for the samples of R. sphaeroides and some of the chromatophores. A number of people, among whom may be mentioned M. Gouterman, D. Mauzerall, W.W. Parson, P.A. Loach, Govindjee, K.J. Kaufmann, C.E. Swenberg, and R.M. Hochstrasser have contributed helpful discussions. Mr. D. Henderson helped with the apparatus. We also thank Dr. William Parson for sending us several of his manuscripts before publication. Financial support came from the National Science Foundation (grants PCM76-15724 and PCM76-23744).

PY - 1978/2/9

Y1 - 1978/2/9

N2 - We have observed fluorescence at visible wavelengths from chromatophores of photosynthetic bacteria excited with infrared radiation which we attribute to bacteriochlorophyll of the antenna system. The fluorescence is prompt (no delay greater than 5 ns). Its spectrum shows peaks at 445, 530 (broad) and 600 nm when excited with either 694 or 868 nm. Quantum yield is of the order of 10-9. The dependence on intensity indicates generation by mainly third-order processes which could involve triplet states in combination with excited singlets. Second-order single-singlet fusion could also contribute. The high-order fluorescence can also be explained as arising from absorption of a second photon by singlet excited states.

AB - We have observed fluorescence at visible wavelengths from chromatophores of photosynthetic bacteria excited with infrared radiation which we attribute to bacteriochlorophyll of the antenna system. The fluorescence is prompt (no delay greater than 5 ns). Its spectrum shows peaks at 445, 530 (broad) and 600 nm when excited with either 694 or 868 nm. Quantum yield is of the order of 10-9. The dependence on intensity indicates generation by mainly third-order processes which could involve triplet states in combination with excited singlets. Second-order single-singlet fusion could also contribute. The high-order fluorescence can also be explained as arising from absorption of a second photon by singlet excited states.

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DO - 10.1016/0005-2728(78)90028-2

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AN - SCOPUS:0018263810

SN - 0005-2728

VL - 501

SP - 217

EP - 231

JO - BBA - Bioenergetics

JF - BBA - Bioenergetics

IS - 2

ER -

High-order fluorescence and exciton interaction in photosynthetic bacteria

Abstract

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