High-resolution 3D MRI of mouse brain reveals small cerebral structures in vivo

O. Natt, T. Watanabe, S. Boretius, J. Radulovic, J. Frahm, T. Michaelis*

*Corresponding author for this work

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

This work demonstrates technical approaches to high-quality magnetic resonance imaging (MRI) of small structures of the mouse brain in vivo. It turns out that excellent soft-tissue contrast requires the reduction of partial volume effects by using 3D MRI at high (isotropic) resolution with linear voxel dimensions of about 100-150 μm. The long T2* relaxation times at relatively low magnetic fields (2.35 T) offer the benefit of a small receiver bandwidth (increased signal-to-noise) at a moderate echo time which together with the small voxel size avoids visual susceptibility artifacts. For measuring times of 1-1.5 h both * relaxation times at relatively low magnetic fields (2.35 T) offer the benefit of a small receiver bandwidth (increased signal-to-noise) at a moderate echo time which together with the small voxel size avoids visual susceptibility artifacts. For measuring times of 1-1.5 h both T1-weighted (FLASH) and T2-weighted (Fast Spin-Echo) 3D MRI acquisitions exhibit detailed anatomical insights in accordance with histological sections from a mouse brain atlas. Preliminary applications address the identification of neuroanatomical variations in different mouse strains and the use of Mn2+ as a T1 contrast agent for neuroaxonal tracing of fiber tracts within the mouse visual pathway.

Original languageEnglish (US)
Pages (from-to)203-209
Number of pages7
JournalJournal of Neuroscience Methods
Volume120
Issue number2
DOIs
StatePublished - Oct 30 2002

Keywords

  • Contrast
  • MR microscopy
  • MRI
  • Manganese
  • Mouse brain
  • Mouse strain
  • Neuroaxonal tracing
  • Phenotyping

ASJC Scopus subject areas

  • Neuroscience(all)

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