TY - GEN
T1 - High resolution acoustic microscopy
T2 - 2007 IEEE Ultrasonics Symposium, IUS
AU - Rupin, F.
AU - Saied, A.
AU - David, V.
AU - Raum, K.
AU - Peyrin, F.
AU - Vico, L.
AU - Laugier, P.
PY - 2007
Y1 - 2007
N2 - In the current study, a 200 MHz scanning acoustic microscope (3.75 μm nominal spatial resolution) has been used to document changes in trabecular bone material properties related to a model of adaptive remodeling. Thirty cylindrical specimens of trabecular bone were extracted from one bovine sternum and one ulna metaphysis. Samples from each bone site (n=15), were separated in 3 groups of 5 samples each. Specimens of C (control) group were immediately fixed after extraction and embedded in methylmetacrylate, NL (no loaded) and L (loaded) groups were maintained viable In a controlled perfusion culture-loading chamber over three weeks. Specimens of L groups underwent a cyclic compressive strain mimicking human jump, whereas NL samples were left free of loading. After the experiment, L and NL samples were fixed and embedded in methylmetacrylate, then all samples were explored with SAM. Z spatial distribution was calculated over the surface of a single trabecular Z mean value was estimated for each sample then, averaged over samples of each group. In controls, Z was significantly higher in ulna than in sternum. The sternum appeared to respond more significantly to mechanical loading than ulna and displayed a significant 30% increase in Z for L group when compared to C group and a 13 % increase when compared to NL group. In conclusion, our results suggest that 400 MHz SAM is relevant to map local changes in bone intrinsic material properties resulting from (i) probable local differences in tissue properties exhibited naturally between weight bearing (ulna) and no bearing (sternum) bones, (ii) heterogeneous adaptive remodeling stimulated by cyclic loading.
AB - In the current study, a 200 MHz scanning acoustic microscope (3.75 μm nominal spatial resolution) has been used to document changes in trabecular bone material properties related to a model of adaptive remodeling. Thirty cylindrical specimens of trabecular bone were extracted from one bovine sternum and one ulna metaphysis. Samples from each bone site (n=15), were separated in 3 groups of 5 samples each. Specimens of C (control) group were immediately fixed after extraction and embedded in methylmetacrylate, NL (no loaded) and L (loaded) groups were maintained viable In a controlled perfusion culture-loading chamber over three weeks. Specimens of L groups underwent a cyclic compressive strain mimicking human jump, whereas NL samples were left free of loading. After the experiment, L and NL samples were fixed and embedded in methylmetacrylate, then all samples were explored with SAM. Z spatial distribution was calculated over the surface of a single trabecular Z mean value was estimated for each sample then, averaged over samples of each group. In controls, Z was significantly higher in ulna than in sternum. The sternum appeared to respond more significantly to mechanical loading than ulna and displayed a significant 30% increase in Z for L group when compared to C group and a 13 % increase when compared to NL group. In conclusion, our results suggest that 400 MHz SAM is relevant to map local changes in bone intrinsic material properties resulting from (i) probable local differences in tissue properties exhibited naturally between weight bearing (ulna) and no bearing (sternum) bones, (ii) heterogeneous adaptive remodeling stimulated by cyclic loading.
KW - Acoustic microscopy
KW - Elastic properties
KW - Impedance
KW - Remodeling
KW - Trabecular bone
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U2 - 10.1109/ULTSYM.2007.280
DO - 10.1109/ULTSYM.2007.280
M3 - Conference contribution
AN - SCOPUS:48149085276
SN - 1424413834
SN - 9781424413836
T3 - Proceedings - IEEE Ultrasonics Symposium
SP - 1109
EP - 1112
BT - 2007 IEEE Ultrasonics Symposium Proceedings, IUS
Y2 - 28 October 2007 through 31 October 2007
ER -