High resolution acoustic microscopy: A new method to investigate remodeling process of trabecular bone

F. Rupin*, A. Saied, V. David, K. Raum, F. Peyrin, L. Vico, P. Laugier

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

In the current study, a 200 MHz scanning acoustic microscope (3.75 μm nominal spatial resolution) has been used to document changes in trabecular bone material properties related to a model of adaptive remodeling. Thirty cylindrical specimens of trabecular bone were extracted from one bovine sternum and one ulna metaphysis. Samples from each bone site (n=15), were separated in 3 groups of 5 samples each. Specimens of C (control) group were immediately fixed after extraction and embedded in methylmetacrylate, NL (no loaded) and L (loaded) groups were maintained viable In a controlled perfusion culture-loading chamber over three weeks. Specimens of L groups underwent a cyclic compressive strain mimicking human jump, whereas NL samples were left free of loading. After the experiment, L and NL samples were fixed and embedded in methylmetacrylate, then all samples were explored with SAM. Z spatial distribution was calculated over the surface of a single trabecular Z mean value was estimated for each sample then, averaged over samples of each group. In controls, Z was significantly higher in ulna than in sternum. The sternum appeared to respond more significantly to mechanical loading than ulna and displayed a significant 30% increase in Z for L group when compared to C group and a 13 % increase when compared to NL group. In conclusion, our results suggest that 400 MHz SAM is relevant to map local changes in bone intrinsic material properties resulting from (i) probable local differences in tissue properties exhibited naturally between weight bearing (ulna) and no bearing (sternum) bones, (ii) heterogeneous adaptive remodeling stimulated by cyclic loading.

Original languageEnglish (US)
Title of host publication2007 IEEE Ultrasonics Symposium Proceedings, IUS
Pages1109-1112
Number of pages4
DOIs
StatePublished - Dec 1 2007
Event2007 IEEE Ultrasonics Symposium, IUS - New York, NY, United States
Duration: Oct 28 2007Oct 31 2007

Publication series

NameProceedings - IEEE Ultrasonics Symposium
ISSN (Print)1051-0117

Other

Other2007 IEEE Ultrasonics Symposium, IUS
CountryUnited States
CityNew York, NY
Period10/28/0710/31/07

Keywords

  • Acoustic microscopy
  • Elastic properties
  • Impedance
  • Remodeling
  • Trabecular bone

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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    Rupin, F., Saied, A., David, V., Raum, K., Peyrin, F., Vico, L., & Laugier, P. (2007). High resolution acoustic microscopy: A new method to investigate remodeling process of trabecular bone. In 2007 IEEE Ultrasonics Symposium Proceedings, IUS (pp. 1109-1112). [4409853] (Proceedings - IEEE Ultrasonics Symposium). https://doi.org/10.1109/ULTSYM.2007.280